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Pig anti-Human IgE Fc Secondary Antibody, Novus Biologicals™

Artikelnummer. 18105022 Shop alle Bio Techne produkter
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Mængde:
10 mg
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18105022 10 mg
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Artikelnummer. 18105022 Leverandør Novus Biologicals Leverandørnr. NBP269114

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Beskrivelse

Pig Polyclonal Antibody

IgE Fc Polyclonal specifically detects IgE Fc in Human samples. It is validated for ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunoblotting, Dot Blot.
TRUSTED_SUSTAINABILITY

Tekniske data

Antigen IgE Fc
Applikationer ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoblot, Dot Blot
Klassifikation Polyclonal
Koncentration LYOPH
Konjugeret Unconjugated
Fortynding ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunoblotting 1:100 - 1:2000, Dot Blot 1:100-1:2000
Formulering Lyophilized from PBS (pH 7.2) with No Preservative
Værtsarter Pig
Immunogen Purified homogenous IgE isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Oprensningsmetode Ion exchange chromatography
Mængde 10 mg
Primær eller sekundær Secondary
Testspecificitet The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule which represents strict isotype (class) specificity. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of IgE, a single precipitin line is obtained which shows a reaction of full identity with the precipitin line obtained with the used immunogen. It does not react with human IgG, including all subclasses, IgA, IgM and IgG/Fab or any non-Ig protein in human serum.
Rekonstitution Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.
Målarter Human
Indhold og opbevaring Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Form Lyophilized
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