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Thermo Scientific™ Pierce™ SILAC Protein Quantitation Kits

Artikelnummer. 11688833 Shop alle Thermo Scientific produkter
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Produkttype:
Protein Quantitation Kit - DMEM:F12
Protein Quantitation Kit - RPMI 1640
Pakningsstørrelse:
1 sæt
Hver
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Artikelnummer. Produkttype unitSize
11688833 Protein Quantitation Kit - DMEM:F12 Hver
10220204 Protein Quantitation Kit - RPMI 1640 1 sæt
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Artikelnummer. 11688833 Leverandør Thermo Scientific™ Leverandørnr. 88439

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Beskrivelse

Analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) using these complete reagent kits.

Thermo Scientific™ SILAC Protein Quantitation Kits are used for the specific analysis of protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC).

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of heavy 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Efficient – 100% label incorporation into proteins of living cells

  • Reproducible – eliminates intra-experimental variability caused by differential sample preparation

  • Flexible – media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through dual amino acid isotope labeling

  • Compatible – label proteins expressed in a wide variety of mammalian cell lines adapted to grow in DMEM, RPMI-1640 or DMEM:F12 medium, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, Jurkat and others

  • Optimized, specialized media – the Expansion DMEM and Low-Osmolarity DMEM are specifically designed and qualified for mouse embryonic stem cell propagation and SILAC protein expression profiling with mass spectrometry

  • High-quality supplements – heavy amino acids with >99% isotope purity; the Stem Cell Screened Dialyzed FBS and Serum Substitute for Mouse Embryonic Stem Cells are tested to ensure that they do not promote stem cell differentiation

Recommended for:

Quantitative analysis of relative changes in protein abundance from different cell treatments; Quantitative analysis of proteins for which there are no antibodies available; Protein expression profiling of normal vs. disease cells; Identification and quantification of hundreds to thousands of proteins in a single experiment

TRUSTED_SUSTAINABILITY

Tekniske data

Formulering Multi-component kit with heavy and light L-Lysine and DMEM:F12 Media
Sættets indhold L-Lysine-2HCl, 50mg, Dialyzed FBS, 2 x 50mL, DMEM:F12 Media for SILAC, 2 x 500mL, L-Arginine-HCl, 2 x 50mg, L-Lysine-2HCl, 13C6, 50mg
Produkttype Protein Quantitation Kit - DMEM:F12
Tilstrækkelig til One complete SILAC experiment (stable isotope labeling with amino acids in cell culture)
Mængde 1 Use Kit
Til brug med (applikation) SILAC experiments (stable isotope labeling with amino acids in cell culture)
Produkttitel
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