Affinity purify antibodies (IgG) with immobilized recombinant Protein G agarose offered in bottles, spin columns, filter plates and complete purification kits.
Brand: Thermo Scientific™ 89949
2180.10 DKK valid until 2025-06-27
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Thermo Scientific™ Pierce Protein G Agarose is a versatile, high-performance affinity resin for antibody purification that is available as bottled agarose beads, pre-packed spin columns, 96-well filter plates and complete IgG purification kits.
Pierce Protein G Agarose is effective for affinity purification of IgG from serum and other fluids of many mammalian species. Protein G is especially well suited for use with mouse antibodies (including IgG1) in addition to most IgG isotypes from human, goat and sheep samples.Pierce Protein G Agarose consists of recombinant Protein G that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose (CL-6B). This premiere variety of the affinity resin provides the most versatile combination of chromatographic features for high yield and high purity purification of whole IgG from mammalian serum samples. The agarose beads have physical and chemical properties suitable for many affinity purification systems. Accordingly, Pierce Protein G Agarose is offered in several convenient package formats, including bottled resin slurries, three sizes of spin columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates.
Highlights:
Protein G – immobilized Protein G is ideal for polyclonal IgG purification from mouse, human, cow, goat and sheep serum, including human IgG3 and mouse IgG1 isotypes
Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable – superior manufacturing method immobilizes Protein G by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
Standard capacity – Pierce Protein G Agarose has a normal load of immobilized Protein G, providing a binding capacity of 11 to 15mg human IgG/mL resin
Multiple formats – choose from bottled resin slurries, centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates
Properties of crosslinked 6% beaded agarose (CL-6B):
Available Formats:
Includes:
Purification kits contain Protein G Spin Columns, binding buffer, elution buffer and neutralization buffer
| Spin Kit | |
| Agarose Resin, Affinity | |
| Protein G | |
| NAb Protein G Spin Purification Kit, 0.2mL | |
| 10 Column Kit |
| NAb™ | |
| Kit, Microcentrifuge Spin Column | |
| IgG Antibodies | |
| Upon receipt store kit at 4°C |
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For Research Use Only. Not for use in diagnostic procedures.
