Purify antibodies or perform immunoprecipitation with this standard-capacity, immobilized native Protein A on beaded agarose resin.
Brand: Thermo Scientific™ 44667
3400.17 DKK valid until 2025-06-27
Use promo code "24112" to get your promotional price.
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price
Perform a variety of antibody affinity purification methods with Thermo Scientific™ Pierce Protein A Agarose, a standard-capacity Protein A beaded agarose resin.
Pierce Protein A Agarose consists of purified native Protein A that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose. The agarose beads have physical and chemical properties that enable them to be used in a variety of batch- or column-type affinity purification procedure. Several package formats are offered: 5mL and 25mL bottles of resin slurry, 1mL pre-packed columns and a complete IgG purification kit.Protein A is a bacteria-derived protein that binds with high affinity and specificity to the Fc portion of antibodies, especially those of the IgG class. Compared to its alternative (Protein G), Protein A provides the highest binding capacity for subclasses of IgG from rabbits, pigs, dogs and cats. Protein A also has higher binding capacity for human IgG, except for IgG3, which it binds weakly. Protein A also binds weakly to mouse IgG1 and is not recommended for most applications with that antibody isotype.
Highlights:
Native Protein A – immobilized Protein A is ideal for polyclonal IgG purification from human, rabbit, pig, dog or cat serum
Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable – superior manufacturing method immobilizes Protein A by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
Standard capacity – Pierce Protein A Agarose has a normal load of immobilized Protein A, providing a binding capacity of 12 to 19mg human IgG/mL resin (approx. 6 to 8mg mouse IgG/mL resin)
Properties of crosslinked 6% beaded agarose (CL-6B):
Available Formats:
Includes:
Kit contains Protein A Column (1mL each), Protein A IgG Binding Buffer, IgG Elution Buffer and desalting columns
| IgG Purification Kit | |
| Agarose Resin, Affinity | |
| Protein A | |
| 6 to 8 mg Mouse IgG per Column | |
| 5 Column Kit |
| Pierce™ | |
| Kit, 15 mL Conical Spin Column | |
| IgG Antibodies | |
| Sufficient For: Purifying 6 to 8 mg mouse IgG per column • Protein A Columns, 1 mL, 5 columns • Protein A IgG Binding Buffer, 1 L • IgG Elution Buffer, 500 mL • Excellulose Desalting Columns, 5 mL, 5 columns Store at 4°C. |
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
For Research Use Only. Not for use in diagnostic procedures.
