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Pierce™ Pierce™ Ni-NTA Magnetic Agarose Beads
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Description
Product features:
Ni-NTA Magnetic Agarose Beads are used for small-scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). Tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates such as those prepared using Thermo Scientific B-PER Bacterial Protein Extraction reagents. Purification of His-tagged proteins is achieved using an NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites that allow for low metal ion leaching and high binding capacity. The protocol for the Ni-NTA Magnetic Agarose Beads has been optimized to allow for high purity of the isolated His-tagged protein.
- High capacity—sufficient for both routine and demanding purification procedures; binds >70 mg of 6xHis-tagged protein per mL of settled resin
- High-performance and reproducible beads—non-aggregating, magnetite (Fe3O4), superparamagnetic beads provide exceptional uniformity for both manual and automated HTS purification applications
- Low nonspecific binding—optimized purification protocol results in better tagged-protein purification
- Versatile—purify proteins using native or denaturing conditions
- Compatible—use with Thermo Scientific Cell Lysis reagents and a variety of buffer additives
Applications:
- Protein purification and isolation
- High throughput protein purification using automated magnetic particle processor
Specifications
Specifications
| Bed Volume | 5 mL |
| Capacity (Metric) | 20 mL |
| Concentration | Slurry: 25% |
| Content And Storage | 5 mL settled resin. Supplied as 20 mL of 25% v/v suspension in 20% ethanol. Store at 2–8°C. |
| Shipping Condition | Wet Ice |
| Particle Size | 30 μm |
| Form | Liquid Suspension |
| Product Line | Pierce™ |
| Quantity | 20 mL |
| Protein Form | Recombinant |
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Documents
Product Information
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Certificates
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Safety and Handling
- Pierce Ni-NTA Magnetic Agaros
- Danger
- Acute toxicity Category 4
- Carcinogenicity Category 2
- Flammable liquid Category 3
- Respiratory sensitiser Category 1B
- Skin sensitiser Category 1B
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H317-May cause an allergic skin reaction.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H351-Suspected of causing cancer.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P403+P235-Store in a well-ventilated place. Keep cool.
- MIXTURE LIST-Contains : ethyl alcohol
For Research Use Only. Not for use in diagnostic procedures.
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