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Thermo Scientific™ Pierce™ High Capacity Streptavidin Chromatography Cartridge, 5 mL Product Code.: 11746436
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Thermo Scientific™ Pierce™ High Capacity Streptavidin Chromatography Cartridge, 5 mL

Product Code. p-7184466 Shop All Thermo Scientific Products
1 cartridge x 5 mL, 5mL
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Quantity:
1 cartridge x 5 mL
2 cartridges x 1 mL
Unit Size:
5mL
Pack of 2
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Regulatory: This product contains a substance listed in Annex XVII to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.

Product Code. 11746436

Brand: Thermo Scientific™ 87740

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This item is not returnable. View return policy
Regulatory: This product contains a substance listed in Annex XVII to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.

Description

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

Thermo Scientific™ Pierce Streptavidin Resins comprise a wide selection of high-quality affinity beads and columns for nearly any kind of biotin-based purification procedure.

Additional Information and Online Ordering

Pierce Streptavidin Agarose and UltraLink Resins supply immobilized streptavidin biotin-binding protein in different resin formats, binding capacities and package sizes to suit nearly any immunoprecipitation or affinity purification procedure involving biotinylated molecules. The products use a recombinant form of streptavidin with a mass of 53 kDa and a near-neutral pI. The resins are prepared by covalently coupling streptavidin to the porous agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in Streptavidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11. The products are excellent choices for a variety of small- or large-scale affinity purification applications involving biotinylated macromolecules, including separation of biotinylated molecules from samples and immunoprecipitation of antigens using biotin-labeled antibodies.

Highlights:

  • Proven coupling chemistries – the methods used to prepare these resins are superior to typical cyanogen bromide (CNBr) immobilization, providing high functionality, low nonspecific binding and excellent leach-resistance

  • Versatile resins – Agarose (polysaccharide-based) and UltraLink™ Biosupport (acrylamide-based) resins provide two different options for particular experimental conditions and are compatible with gravity-flow, spin and FPLC methods

  • Available in multiple formats – choose from two resin types, regular or high capacity preparations, and small or large-scale package sizes (bulk sizes are also available)

  • Superior performance – High Capacity Resin has 2 to 3 times higher binding capacity than alternative supplier products, allowing use of small amounts of resin for experiments

Item-Specific Features:

  • Agarose Resins are crosslinked 6% beaded agarose
  • UltraLink Resins beaded acrylamide support (UltraLink Biosupport)
  • Plus and High Capacity Resins have higher loading (i.e. density) of immobilized streptavidin protein than the regular forms

Recommended for:

Purification of membrane antigens in conjunction with biotinylated monoclonal antibodies; Cell-surface labeling with biotinylation reagents, followed by precipitation with immobilized streptavidin; Purification of cell-surface glycoproteins using biotinylated Concanavalin A; Recovery of single-stranded DNA for dideoxy sequencing

TRUSTED_SUSTAINABILITY

Specifications

Capacity (Metric) 5 mL
Content And Storage Upon receipt store at 4°C. Do not freeze the resin. Product is shipped at ambient temperature.
Particle Size 45 to 165 ÎĽm
Product Line Pierce™
Quantity 1 cartridge x 5 mL
Protein Form Recombinant
Target Biotin-Tagged Molecule
Type Chromatography Cartridge
Stationary Phase Streptavidin
Sufficient For Binding ≥/= 500 μg Biotin per Cartridge (≥/= 50 mg Biotinylated BSA per Cartridge)
Format Chromatography Cartridge
Column Type Affinity, Agarose Resin
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Lot NumberZE394729 Certificate TypeCertificate of Analysis Date31/05/2024 Product Code87740
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