Learn More
Thermo Scientific™ Pierce™ FITC Antibody Labeling Kit
Shop All Thermo Scientific ProductsDescription
Thermo Scientific™ FITC and Fluorescein Labeling Reagents and Kits are high-performance derivatives of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.
Fluorescein dyes are used in wide-ranging applications including fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. FITC and NHS-fluorescein are reactive toward primary amine groups on proteins, peptides and other biomolecules, while fluorescein-5-maleimide and 5-IAF react with free sulfhydryls on cysteine residues.Most activated fluorescein derivatives are mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.
Highlights:
Amine-specific labeling: the NHS-ester or isothiocyanate efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Optimized procedure: following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification: kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery
Specifications
Specifications
| Description | Pierce FITC Antibody Labeling Kit |
| Product Type | Labeling Kit |
| Quantity | 3 reactions kit |
| Sufficient For | Labeling and purifying 3 x 1 mg (2 mg/mL) of IgG or other proteins |
| Content And Storage |
|
| Excitation/Emission | 494/518 |
| Labeling Scale | 0.5 to 1 mg |
| Product Line | Pierce™ |
| Solubility | Aqueous Buffer |
| Label or Dye | FITC (Fluorescein) |
| Show More |
Documents
Product Information
Manuals
Certificates
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
For Research Use Only. Not for use in diagnostic procedures.
Your input is important to us. Please complete this form to provide feedback related to the content on this product.