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Thermo Scientific™ Fluorescein-5-Maleimide

Artikelnummer. 10078847 Shop alle Thermo Scientific produkter
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Mængde:
25 mg
Pakningsstørrelse:
25mg
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Artikelnummer. 10078847

Brand: Thermo Scientific™ 62245

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Beskrivelse

Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).

Thermo Scientific™ FITC and Fluorescein Labeling Reagents and Kits are high-performance derivatives of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

Fluorescein dyes are used in wide-ranging applications including fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. FITC and NHS-fluorescein are reactive toward primary amine groups on proteins, peptides and other biomolecules, while fluorescein-5-maleimide and 5-IAF react with free sulfhydryls on cysteine residues.Most activated fluorescein derivatives are mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.

Highlights:

  • Amine-specific labeling: the NHS-ester or isothiocyanate efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Optimized procedure: following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification: kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery
TRUSTED_SUSTAINABILITY

Tekniske data

Mængde 25 mg
Produkttype Maleimide
Opløselighed DMF (Dimethylformamide)
Indhold og opbevaring Store desiccated at -20°C, protect from moisture
Kemisk reaktivitet Thiol
Reaktiv del Maleimide
Etiket eller farvestof FITC (Fluorescein)
Etikettype Classic Dyes
Produktlinje Pierce
Emission 518
Excitation 494
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Sikkerhed og håndtering

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Produktidentifikator
  • Fluorescein-5-Maleimide, 25 mg
 Signalord
  • Advarsel
 Farekategori
  • Akut toksicitet Kategori 4
 Faresætning
  • H302-Farlig ved indtagelse.
 Sikkerhedssætning
  • P270-Der må ikke spises, drikkes eller ryges under brugen af dette produkt.
  • P264-Vask grundigt efter brug.
  • P301+P312-I TILFÆLDE AF INDTAGELSE: I tilfælde af ubehag ring til en GIFTINFORMATIO / læge /
  • P330-Skyl munden.
  • P501-Indholdet/beholderen bortskaffes i
 Supplerende oplysninger
  • MIXTURE LIST-Indeholder: Fluorescein-5-maleimide

For Research Use Only. Not for use in diagnostic procedures.

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