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Thermo Scientific™ Pierce™ Fab Preparation Kit
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Description
Thermo Scientific™ Pierce Fab Preparation Kits use immobilized papain protease to digest human or mouse IgG antibodies to make separate Fab and Fc fragments and subsequently to purify the Fab using Protein A agarose.
These kits use papain, a nonspecific thiol-endopeptidase, to enzymatically cleave whole IgG just above the hinge region to create two separate Fab fragments and one Fc fragment per antibody molecule. Because the papain protease is supplied in immobilized form as beaded agarose resin, the digestion reaction is easily stopped by removing the resin from the IgG solution; the result is digest products that are enzyme-free. The antibody digestion reaction is performed in convenient disposable spin columns that allow efficient removal of the immobilized protease and maximum recovery of the IgG fragments. Also included in the kits are Protein A spin columns and buffers to efficiently purify the resulting fragments. Protein A binds the Fc fragments and undigested IgG, allowing the pure Fab fragments to be recovered in the flow-through fraction. Thermo Scientific Zeba™ Desalting Spin Columns make IgG-sample preparation quick and easy.
Highlights:
Enzyme-free digestion products – immobilized papain (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the protease
Suitable for human and other species of IgG – the kit procedure is optimized for human, mouse and rabbit IgG, but papain-based digestion is effective for many species and subclasses of IgG including rat, goat and pig (Note: for best results with mouse IgG1, use Part No. 44980)
High capacity – kit provides for digestion and purification from as much as 4mg of IgG at a time. Use the standard size kit for fragmenting 0.25 to 4mg IgG, and the micro kit for 25 to 250μg IgG
Provides ready-to-use Fab – digestion and final recovery of purified Fab fragments occurs in neutral pH sodium phosphate buffer, suitable for storage or immediate use in typical applications
Complete and easy to use – kits include all reagents, spin columns and accessories needed to prepare and purify antibody fragments
Includes:
Papain agarose resin, cysteine, digestion buffer, Protein A columns with binding and elution buffers, spin desalting columns and accessories
Specifications
Specifications
| Description | Pierce Fab Preparation Kit |
| Quantity | 10 Antibody Samples, each containing 0.25 to 4 mg IgG kit |
| Product Line | Pierce™ |
| Content And Storage | Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG • Pierce Papain Agarose, 1.25 mL • Cysteine-HCl, 1 g • Fab Digestion Buffer, 120 mL • NAb Protein A Plus Spin Column, 1 mL, 1 column • PBS Packs (each makes 500 mL), 2 packs • IgG Elution Buffer, 120 mL • Zeba Desalt Spin Columns, 7K MWCO, 2mL, 10 columns • Spin Columns, 0.8 mL, 10 columns • Microcentrifuge Tubes, 2 mL, 30 tubes Store at 4–8°C. |
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Safety and Handling
- Pierce Fab Preparation Kit
- Danger
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H335-May cause respiratory irritation.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P284-¢In case of inadequate ventilation! wear respiratory protection.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Sodium chloride, Sodium phosphate dibasic, Papain, L-(+)-Cysteine hydrochloridemonohydrate
For Research Use Only. Not for use in diagnostic procedures.
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