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Invitrogen™ pHrodo™ Antistofmærkningssæt
Beskrivelse
Løft biofarmaceutisk forskning med pHrodo Antistofmærkningssæt, designet til at karakterisere antistofinternalisering, et kritisk trin under forskning og udvikling af antistof-lægemiddelkonjugat. Kits giver en enkel, detaljeret protokol og avancerede oprensningskolonner for nemt at mærke og oprense antistoffer og andre store proteiner. Amin-reaktiv pHrodo farvestoffer binder kovalent til antistoffer og andre proteiner for at producere lyse, opløselige konjugater, hvis fluorescens stiger i sure organeller, herunder endosomer og lysosomer. Antistofinternalisering kan evalueres ved hjælp af flowcytometri, fluorescensmikroskopi og højindholdsscreening.
The pHrodo Red Antibody Labeling Kit and pHrodo Green Antibody Labeling Kit provide the reagents needed to easily label three purified protein or antibody samples with pHrodo Red or pHrodo Green dye. These kits contain amine-reactive, pH-sensitive pHrodo Red or Green STP ester dye that covalently binds to free lysines anywhere they occur in an antibody or protein.
The dyes included in this kit are improved versions of pHrodo Red and pHrodo Green dyes. They are optimized for the creation of bright and soluble bioconjugates that are used in the study of antibody internalization, endocytosis, phagocytosis, and for development of antibody-drug conjugates. These dyes are also more soluble than the original pHrodo Red and pHrodo Green dyes, making them useful for the labeling of antibodies that may otherwise precipitate out of solution during conjugation. pHrodo Red and pHrodo Green dyes are non-fluorescent outside the cell, but fluoresce bright red or bright green as the pH of their surroundings becomes more acidic, such as those found inside of phagosomes and endosomes.
The pHrodo Red and Green Microscale Labeling Kits offer the following benefits:
• Convenience—Labeling typically takes one hour, with less than 30 minutes of hands-on time
• Versatility—Labelling can be performed on 12-150 kDa molecular weight proteins
• Customization—Create your own probes for studying antibody internalization, phagocytosis, and endocytosis
Tekniske data
Tekniske data
| Til brug med (applikation) | Flow Cytometry, Fluoresence Microscopy |
| Produkttype | Antibody Labeling Kit |
| Mængde | 3 x 100 μg |
| Tilstrækkelig til | 3 x 100 μg Reactions |
| Indhold og opbevaring | Store refrigerated. |
| Detektionsmetode | Fluorescence |
| Excitation/Emission | 560/585 nm |
| Mærkningsskala | 100 μg |
| Produktlinje | pHrodo |
| Kemisk reaktivitet | Amine |
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