missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Buffers and Standards
  4. General Chemistry Solutions
  5. Thermo Scientific Chemicals Phenol:Chloroform:Isoamyl alcohol 25:24:1, Ready-to-Use saturated aq. soln., pH 5.2
  6. 15405805

Thermo Scientific Chemicals Phenol:Chloroform:Isoamyl alcohol 25:24:1, Ready-to-Use saturated aq. soln., pH 5.2

Phenol-Chloroform-Isoamyl alcohol mixture in the ratio of 25:24:1 (v/v/v), CAS # 136112-00-0, 67-66-3, 123-51-3, is a solution used in the purification of nucleic acids.

Brand:  Thermo Scientific Chemicals J62336.AN

3275.36 DKK valid until 2025-12-31
Use promo code "25339" to get your promotional price.

Quantity 400 mL

 View more versions of this product

Regulatory: This product fall under regulation “Lov om kemiske stoffer og produkter - lovbekendtgørelse nr. 1755 af 22. december 2006. If applicable, customers must provide a license before delivery.

Product Code. 15405805

  • 3295.00 DKK / 400mL

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Description

Phenol:Chloroform:Isoamyl alcohol 25:24:1 is used in the extraction of DNA for the generation of DNA minisatellite fingerprints. It is also used for purifying RNA.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

  • This solution contains a mixture of pure chloroform, isoamyl alcohol and phenol saturated with Tris-EDTA, with a stabilized pH of 8.0

Applications

  • Phenol-Chloroform-Isoamyl alcohol mixture solution (25:24:1) is used in the extraction of nucleic acids, DNA and RNA
  • When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.
  • It is also used to generate DNA minisatellite fingerprints
TRUSTED_SUSTAINABILITY
Specifications
Show More
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Promotions

Promotions

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title
Thermo Scientific Chemicals Phenol:Chloroform:Isoamyl alcohol 25:24:1, Ready-to-Use saturated aq. soln., pH 5.2 > 400mL

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.