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OPTIZYME™ PvuI, Fisher BioReagents™

Name: OPTIZYME&trade; PvuI, Fisher BioReagents&trade;
Brand: Fisher Bioreagents
SKU: 11904812
Price: 865.00 EUR

Brand: Fisher Bioreagents BP8050-1

Additional Details : Weight : 0.01000kg

Product Code. 11904812

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Description

5'...C G A T^C G...3'
3'...G C^T A G C...5'

Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:

1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl₂, 100mM KCl and 0.1mg/ml BSA
Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

Storage Buffer:

10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 90% of the DNA fragments can be ligated and more than 95% of these can be re-cut after 50-fold over-digestion with PvuI.

Digestion of Agarose-embedded DNA:

A minimum of 5 units of PvuI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.

Compatible Ends: Bsp119I, Bsu15I, Hin1I, Hin6I, HpaII, MaeII, MspI, NarI, Psp1406I, SsiI, XmiI

Methylation Effects:

Dam: Completely overlaps - no effect

(No effect on cleavage)

Dcm: Never overlaps - no effect

CpG: Completely overlaps - blocked

(Cleavage is blocked)

EcoKI: Never overlaps - no effect

EcoBI: Never overlaps - no effect

Specifications

Concentration	10 U/μL
Incubator Temperature	37°C
For Use With (Application)	>90% of DNA fragments can be ligated and re-cut after 10-fold over-digestion with Pvu I
Content And Storage	Keep container tightly closed
Cut Site	CGAT.CG
Form	Liquid

Components	>50% Glycerin, 25 to 50% Water, 1 to 2.5% Sodium Chloride
pH	7.4
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 300mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
Quantity	300 U
Product Type	PvuI

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