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OPTIZYME™ NheI, Fisher BioReagents™
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Mængde:
500 U
Pakningsstørrelse:
Hver
Beskrivelse
5'...G^C T A G C...3'
3'...C G A T C^G...5'
Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 100%
- Buffer 2: 20 - 50%
- Buffer 3: 0 - 20%
- Buffer 4: 100%
- Buffer 5: 0 - 20%
- 10mM Tris-HCl (pH 8.0 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
- Low salt, high glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.
- NheI is inhibited by salt concentrations above 100mM.
- Supercoiled plasmids may require up to 10-fold more NheI for complete digestion than linear DNAs (e.g. 10 units are required to cleave 1μg of pBR322 DNA).
Methylation Effects:
Dam: Never overlaps - no effect
Dcm: Never overlaps - no effect
CpG: May overlap - cleavage impaired
- 5'...GCTAGm5C G...3'
- 5'...m5C GCTAGm5C G...3'
Tekniske data
Tekniske data
| Koncentration | 10 U/μL |
| Komponenter | 25 to 50% Water, >50% Glycerin |
| pH | 7.4 |
| Til brug med (applikation) | >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Nhe I |
| Opbevaringsbuffer | 10mM Tris HCl (pH 8 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol |
| Indhold og opbevaring | Keep container tightly closed |
| Mængde | 500 U |
| Klip websted | G.CTAGC |
| Produkttype | NheI |
| Form | Liquid |
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