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OPTIZYME™ DdeI (HpyF31), Fisher BioReagents™
3025.00 DKK
Specifications
| Concentration | 10 U/μL |
|---|---|
| Components | 25 to 50% Water, >50% Glycerin |
| Incubator Temperature | 37°C |
| pH | 7.4 |
| For Use With (Application) | >95% of DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI |
Description
5'...C^T N A G...3'
3'...G A N T^C...5'
Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
- Incubate at 37°C
- Enzyme Activity in OPTIZYME buffers:
- Buffer 1: 20 - 50%
- Buffer 2: 20 - 50%
- Buffer 3: 20 - 50%
- Buffer 4: 100%
- Buffer 5: 20 - 50%
- 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycero
- Ligation and Re-cleavage:
- More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI.
- Compatible Ends: BbvCI, Bpu10I, Bpu1102I, Eco81I
- Methylation Effects:
- Dam: Never overlaps - no effect
- Dcm: Never overlaps - no effect
- CpG: Never overlaps - no effect
- EcoKI: Never overlaps - no effect
- EcoBI: May overlap - effect not determined
Storage Buffer:
Specifications
| 10 U/μL | |
| 37°C | |
| >95% of DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI | |
| Keep container tightly closed | |
| Liquid |
| 25 to 50% Water, >50% Glycerin | |
| 7.4 | |
| 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol | |
| C.TNAG |