missing translation for 'onlineSavingsMsg'
Få mere at vide
  1. Home
  2. Products
  3. Enzymer og hæmmere
  4. Restriktionsenzymer
  5. OPTIZYME™ DdeI (HpyF31), Fisher BioReagents™

OPTIZYME™ DdeI (HpyF31), Fisher BioReagents™

Artikelnummer. 11984812
Skift visning
Klik for at se tilgængelige muligheder
Mængde:
500 enheder
Produkttype:
DdeI
Pakningsstørrelse:
Hver
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Artikelnummer. Mængde Produkttype unitSize
11984812 500 enheder DdeI Hver
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Denne vare kan ikke returneres. Se returpolicy
Artikelnummer. 11984812 Leverandør Fisher BioReagents Leverandørnr. BP80601
Request Bulk or Custom Format

Venligst for at købe denne vare. Brug for en webkonto? Register dig hos os i dag!


 Se alternative produkter

Denne vare kan ikke returneres. Se returpolicy

Beskrivelse

5'...C^T N A G...3'
3'...G A N T^C...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
  • Incubate at 37°C
  • Enzyme Activity in OPTIZYME buffers:
    • Buffer 1: 20 - 50%
    • Buffer 2: 20 - 50%
    • Buffer 3: 20 - 50%
    • Buffer 4: 100%
    • Buffer 5: 20 - 50%

    Storage Buffer:
    • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycero
    l
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI.
  • Compatible Ends: BbvCI, Bpu10I, Bpu1102I, Eco81I
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Never overlaps - no effect
  • EcoKI: Never overlaps - no effect
  • EcoBI: May overlap - effect not determined

TRUSTED_SUSTAINABILITY

Tekniske data

Koncentration 10 U/μL
Komponenter 25 to 50% Water, >50% Glycerin
Inkubator temperatur 37°C
pH 7.4
Til brug med (applikation) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI
Opbevaringsbuffer 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Indhold og opbevaring Keep container tightly closed
Mængde 500 enheder
Klip websted C.TNAG
Produkttype DdeI
Form Liquid
Vis mere Vis mindre
Produkttitel
Vælg et problem

Ved at klikke på Send, anerkender du, at du kan blive kontaktet af Fisher Scientific med hensyn til den feedback, du har givet i denne formular. Vi deler ikke dine oplysninger til andre formål. Alle angivne kontaktoplysninger skal også vedligeholdes i overensstemmelse med vores Privatlivspolitik.