missing translation for 'onlineSavingsMsg'
Få mere at vide
  1. Home
  2. Products
  3. Enzymer og hæmmere
  4. Restriktionsenzymer
  5. OPTIZYME™ BamHI, Fisher BioReagents™

OPTIZYME™ BamHI, Fisher BioReagents™

Artikelnummer. 11974842
Klik for at se tilgængelige muligheder
Mængde:
12,500 U
Pakningsstørrelse:
Hver
Denne vare kan ikke returneres. Se returpolicy

Artikelnummer. 11974842

Brand: Fisher BioReagents BP80055

Request Bulk or Custom Format

Venligst for at købe denne vare. Brug for en webkonto? Register dig hos os i dag!


 Se alternative produkter

Denne vare kan ikke returneres. Se returpolicy

Beskrivelse

5'...G^G A T C C...3'
3'...C C T A G^G...5'

Conditions for 100% Activity:

  • 1X OPTIZYME Buffer BamHI: 10mM Tris-HCl (pH 8.0 at 37°C), 5mM MgCl2, 100mM KCl, 0.02% Triton X-100 and 0.1mg/ml BSA.
  • Incubate at 37°C
Supplied with: 10X OPTIZYME BamHI Buffer
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 20 - 50%™
  • Buffer 2: 100%
  • Buffer 3: 20 - 50%
  • Buffer 4: 100%™
  • Buffer 5: 50 - 100%™
™star activity occurs at greater than 5-fold overdigestion (5 units × 1 hour)
Storage Buffer:
10mM Tris-HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with BamHI.
Digestion of Agarose-embedded DNA:
A minimum of 5 units of BamHI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Note: Low salt, high glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity.
Compatible Ends: BclI, BglII, Bsp143I, MboI, PsuI.
Methylation Effects:
Dam: completely overlaps - no effect.
Dcm: may overlap - no effect.
CpG: may overlap - no effect.
EcoKI: never overlaps - no effect.
EcoBI: never overlaps - no effect.

TRUSTED_SUSTAINABILITY

Tekniske data

Koncentration 10 U/μL
Komponenter 25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
Inkubator temperatur 37°C
pH 7.4
Til brug med (applikation) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BamHI
Opbevaringsbuffer 10mM Tris HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
Indhold og opbevaring Keep container tightly closed
Mængde 12,500 U
Klip websted G.GATCC
Produkttype BamHI
Form Liquid
Vis mere Vis mindre
Korrektion af produktindhold

Dit input er vigtigt for os. Udfyld denne formular for at give feedback relateret til indholdet på dette produkt.

Produkttitel

Ved at klikke på Send, anerkender du, at du kan blive kontaktet af Fisher Scientific med hensyn til den feedback, du har givet i denne formular. Vi deler ikke dine oplysninger til andre formål. Alle angivne kontaktoplysninger skal også vedligeholdes i overensstemmelse med vores Privatlivspolitik.

Mange tak! Din feedback er blevet sendt. Hos Fisher Scientific arbejder vi altid på at forbedre vores indhold for dig. Vi sætter pris på din feedback.