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OPTIZYME™ AluI, Fisher BioReagents™

Artikelnummer. 11904782
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Mængde:
600 U
Pakningsstørrelse:
Hver
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11904782 600 U Hver
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Artikelnummer. 11904782 Leverandør Fisher BioReagents Leverandørnr. BP80151
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Beskrivelse

5'...A G^C T...3'
3'...T C^G A...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 50 - 100%
  • Buffer 2: 0 - 20%
  • Buffer 3: 0 - 20%
  • Buffer 4: 100%
  • Buffer 5: 0 - 20%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1 mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with AluI
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Never overlaps - no effect
  • EcoKI: Never overlaps - no effect
  • EcoBI: May overlap - blocked
    • 5'...TGm6AGCT(N)5 TGCT...3'
  • 3'...AC TCGA(N)5 m6ACGA...5'
  • (Cleavage is blocked)

    • TRUSTED_SUSTAINABILITY

    Tekniske data

    Koncentration 10 U/μL
    Komponenter 25 to 50% Water, >50% Glycerin
    Inkubator temperatur 37°C
    pH 7.4
    Til brug med (applikation) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with AluI
    Opbevaringsbuffer 10mM Tris HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
    Indhold og opbevaring Keep container tightly closed
    Mængde 600 U
    Klip websted AG.CT
    Produkttype Alul
    Form Liquid
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    Produkttitel
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