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  6. Macherey-Nagel™ NucleoSpin™ TriPrep, Mini kit for RNA, DNA, and Protein Purification

Macherey-Nagel™ NucleoSpin™ TriPrep, Mini kit for RNA, DNA, and Protein Purification

Artikelnummer. 11962502
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Tilstrækkelig til:
10rxns
250rxns
Pakningsstørrelse:
10 reaktioner
250 reaktioner
50 reaktioner
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Artikelnummer. 11962502

Brand: Macherey-Nagel™ 740966.10

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Beskrivelse

Parallel isolation of RNA, DNA and, protein from an undivided sample.

NucleoSpin™ miRNA is suitable for the parallel isolation of small RNA, large RNA and protein.

Superior selectivity - purify your RNA fractionated by size

Isolation of small RNA only (18 to 200 bases)Isolation of small (18 to 200 bases) and large (>200 bases) RNA in two fractionsIsolation of total RNA (small and large in one fraction)

Additional isolation of total protein

Denatured protein ready-to-use for analysis in SDS-PAGE and Western blot.

Excellent RNA recovery - even without phenol/chloroform

Lysis with chaotropic saltSpin column based procedure

Convenient handling - complete equipment provided

NucleoSpin filters - efficient sample homogenisationNucleoSpin protein removal columns - highest purity of small RNArDNase - highly efficient on-column digestion of genomic DNA at RT

  • Silica membrane technology
  • Format: minispin columns
  • Sample size: <107 cultured cells, <30mg human/tissue; <50 mg plant tissue, <150μL reaction mixture
  • Fragment size, small RNA: 18 to 200 bases
  • Fragment size, large RNA: >200 bases
  • Typical yield: 10μg small RNA; 95μg large RNA from 107 HeLa cells
  • Binding capacity: 200μg
  • Elution volume, 30μL to 100μL
  • Preparation time: <45min (6 preps human/animal tissue, small and large RNA); <35min (6 preps human/animal tissue, small RNA only)
  • Small RNA (18 to 200 bases)
  • Large RNA (>200 bases)
  • Total RNA (small and large RNA in one fraction), and isolate your total protein fraction For small and large RNA and total RNA purification, and isolation of total protein fraction.

Tekniske data

Produkttype NucleoSpin™ TriPrep Column Set
Mængde 1 Set
Inkluderer 2mL Collection Tubes, 1.5mL Collection Tubes, NucleoSpin™ Filters, buffers, RNase-free and rDNase
Tilstrækkelig til 10rxns
Kolonnetype Spinsøjle
Til brug med (applikation) For Rapid Purification of Total RNA, DNA, and Protein From Small and Precious Samples

Sikkerhed og håndtering

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Farekategori
  • Respiratorisk sensibiliserende stof Kategori 1
  • Hudsensibilisering Kategori 1
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