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Macherey-Nagel™ NucleoSpin™ RNA Plus, Mini kit for RNA purification with DNA removal column
Beskrivelse
During the isolation of RNA, it is imperative to prevent the degradation of the RNA. In the NucleoSpin™ RNA Plus method, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases. The lysate is added to the gDNA Removal Column to clarify the lysate and to remove contaminating gDNA. After the addition of the Binding Solution to the flow-through, the RNA is bound to the RNA Plus Column. Two subsequent wash steps remove salts, metabolites, and macromolecular cellular components. High quality RNA is eluted with RNase-free H2O.
- Includes NucleoSpin™ gDNA Removal Column: no time consuming rDNase digestion
- Convenient handling: lysate clearing and gDNA removal with one column in one step
- New efficient lysis buffer: no β-mercaptoethanol or TCEP necessary
- Downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
- Kit contents: NucleoSpin RNA Plus Columns, NucleoSpin gDNA Removal Columns, Collection Tubes, buffers
Tekniske data
Tekniske data
| Bakterieindhold | <109 Cells |
| Indhold og opbevaring | Room temperature (18-25°C) |
| Buffer | Lyseringsbuffer |
| Format | Mini spinsøjle |
| Isoleringsteknologi | Silica Membrane System (Two Columns) |
| Elueringsvolumen | Elution volume: 30–120μL |
| Prøvetype | <1 x 107 Cultured Cells, <1 x 109 Bacterial Cells, <1 x 108 Yeast Cells, <30 mg Human/Animal Tissue |
| Til brug med (applikation) | RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms |
| Inkluderer | NucleoSpin™ RNA Plus Columns with Collection Tubes, NucleoSpin™ gDNA Removal Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers |
| Antal reaktioner | 10 |
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Sikkerhed og håndtering
- NucleoSpin RNA Plus
- Advarsel
- Brandfarlig væske Kategori 3
- Akut toksicitet Kategori 4
- LANGTIDSFARE FOR VANDMILJØET Kronisk kategori 3
- H226-Brandfarlig væske og damp.
- H302-Farlig ved indtagelse.
- H412-Skadelig for vandlevende organismer, med langvarige virkninger.
- MIXTURE LIST-Indeholder: ethyleneglycol diacetate / guanidinium thiocyanate /guanidine hydrochloride, ethanol
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