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Macherey-Nagel™ NucleoSpin™ RNA Plant, Mini kit for RNA from plant
Beskrivelse
NucleoSpin™ RNA Plant Kit is designed for isolation of DNA-free, high-quality RNA from a wide variety of plant and fungal samples. Typical downstream applications include real-time RT-PCR, gene expression profiling, Northern blotting, primer extension, array technology, and RNase protection assays.
- Two different lysis buffers included—Optimized lysis procedure for typical conditions and for peculiar metabolites
- rDNase included for on-column digestion—Efficient removal of contaminating DNA
- NucleoSpin™ Filters (shredders) included—Efficient sample homogenization and reduction of viscosity
- Parallel purification of DNA possible—By using the NucleoSpin™ RNA/DNA buffer set
Kit Components: NucleoSpin™ RNA columns with 2 mL collection tubes, 2 mL and 1.5 mL collecting tubes, NucleoSpin™ filters, buffers (lysis, wash, desalting), RNase-free DNase I, reaction buffer for DNase, RNase-free water
- Mini spin column with silica membrane technology
- Yields 3 to 70μg RNA from 100μg plant material
- Elution volume: 40 to 60μL
- Fragment size: 200b–20kb
- Binding capacity: 200μg
- Preparation time: 30 min./6 preps
- A260/A280: 1.9–2.1
Tekniske data
Tekniske data
| Format | Mini spinsøjle |
| Til brug med (udstyr) | Real-time RT-PCR |
| Til brug med (applikation) | RNA purification from plant cells and tissue |
| Inkluderer | Lysis buffer RA1 (10mL), Lysis buffer RAP (10mL), Wash buffer RAW2 (13mL), Wash Buffer RA3 concentrate (6mL), Membrane desalting buffer (10mL), Reaction buffer for rDNase (7mL), RNase-free rDNase (1 size D vial), RNase-free water (13mL), NucleoSpin filters (10), NucleoSpin RNA Plan Columns with collection tubes (10), 2mL Collection tubes (30), 1.5mL Collection tubes (10) |
| Antal reaktioner | 10 forberedelser |
| Produkttype | NucleoSpin™ RNA Plant Column |
| Mængde | 1 stk. |
| Prøvestørrelse | <100 mg |
| Udbytte | 3–70μg |
Sikkerhed og håndtering
- NucleoSpin RNA Plant
- Fare
- Brandfarlig væske Kategori 3
- Akut toksicitet Kategori 4
- Hudætsning/hudirritation Kategori 2
- Alvorlige øjenskader/øjenirritation Kategori 2
- Respiratorisk sensibiliserende stof Kategori 1
- LANGTIDSFARE FOR VANDMILJØET Kronisk kategori 3
- H226-Brandfarlig væske og damp.
- H302-Farlig ved indtagelse.
- H315-Forårsager hudirritation.
- H319-Forårsager alvorlig øjenirritation.
- H334-Kan forårsage allergi- eller astmasymptomer eller åndedrætsbesvær ved indånding.
- H412-Skadelig for vandlevende organismer, med langvarige virkninger.
- P261-Undgå indånding af pulver/røg/gas/tåge/damp/spray.
- P342+P311-Ved luftvejssymptomer: Ring til en GIFTINFORMATION/læge/ .
- MIXTURE LIST-Indeholder: ethanol (diluted < 20 %)(denatured with1%IPA/1%MEK, acc.2016/1867/EU); guanidinium thiocyanate; gua
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