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Macherey-Nagel™ NucleoSpin™ RNA Columns, Mini columns for RNA purification
Beskrivelse
NucleoSpin™ RNA
- rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
- NucleoSpin Filters (shredders) – efficient sample homogenization and reduction of viscosity
- Up to 70 μg ready to use RNA
- Parallel purification of genomic DNA by using the NucleoSpin™ RNA/DNA Buffer Set
- RNA isolation from cultured cells, tissue (standard protocol):
- RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
- RNA from ≤ 100 μL biological fluids
- RNA clean-up from reaction mixtures
- RNA from samples stored in RNAlater™
- RNA from saliva samples collected with Oragene™ RNA (Genotek)
- Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
Tekniske data
Tekniske data
| Farve | Blue band |
| Indhold og opbevaring | rDNase at 4 °C; Other components at room temperature (18°C to 25 °C) |
| Format | Mini spinsøjle |
| Isoleringsteknologi | Silica membranteknologi |
| Elueringsvolumen | 40 to 120 μL |
| Prøvetype | Cultured Bacterial or Yeast Cells, Human or Animal Tissue |
| Effektivitet | Binding capacity: 200 μg |
| Til brug med (udstyr) | NucleoSpin RNA Kits |
| Til brug med (applikation) | Isolation of RNA from cells and tissue |
| Inkluderer | Columns only |
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Sikkerhed og håndtering
Produktidentifikator
- NucleoSpin RNA
- MIXTURE LIST-Indeholder: guanidinium thiocyanate, ethanol (diluted < 20 %) (denaturedwith 1%IPA/1%MEK, acc.2016/1867/EU); gu
Produkttitel
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