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Macherey-Nagel™ NucleoSpin™ Plasmid Transfection-grade, Mini kit for ultrapure plasmid DNA

Product Code. 15593626
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Product Code. 15593626 Supplier Macherey-Nagel™ Supplier No. 740490.250
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Description

A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Complete kit includes mini spin columns for an ultra-fast workflow.

Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.

  • Purifies high-copy plasmids for transfection
  • Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
  • Rapid purification from E. coli via manual, vacuum or centrifugation processing
  • Ultra-fast procedure using mini spin columns simplifies the work-flow
  • Downstream applications: transfection of common cell lines (lipofection, electroporation)
  • Also suitable for standard molecular biology application, such as cloning and sequencing

Specifications

Bakterielle endotoksiner <50 EU/ng DNA
Indhold og opbevaring Room temperature (18 - 25 °C)
Format Mini spinsøjle
Isoleringsteknologi Silica Membrane and Endotoxin-reduction Technology
Prøvetype Bacterial Culture (E. coli, High-copy Plasmids)
Effektivitet Binding capacity: 35 μg
Elueringsvolumen Elution Volume: 30 - 50 μL
Endelig produkttype Transfection-grade plasmid DNA
Til brug med (udstyr) Centrifuge, Vacuum manifold (e.g. NucleoVac 96)
Til brug med (applikation) Rapid purification of plasmids for transfection
Inkluderer NucleoSpin Plasmid Transfection-grade Columns, Collection Tubes, Buffers, RNase A
Antal prøver 250
Renhed A260/A280: 1.80 - 1.85
Prøvevolumen Up to 5 mL
Størrelse < 15 kbp
Test tid 14 min/6 preps
Udbytte 15 - 30 μg
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