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Macherey-Nagel™ NucleoSpin Plasmid QuickPure™ Kit
Beskrivelse
With the NucleoSpin™ Plasmid QuickPure method, the bacteria culture is lysed according to alkaline lysis. The lysate is cleared by centrifugation and the supernatant is loaded onto the NucleoSpin™ Plasmid QuickPure column. Contaminations like salts, metabolites, nucleases and soluble macromolecular cellular components are removed by only one single washing step with buffer AQ. Pure plasmid DNA is finally eluted under low ionic strength conditions with slightly alkaline buffer AE (5mM Tris-Cl, pH 8.5). The prepared plasmid DNA is suitable for all common downstream applications.
Kit components:
Resuspension Buffer, Lysis Buffer, Neutralization Buffer, Wash Buffer, Elution Buffer, RNase A, NucleoSpin™ Plasmid QuickPure Columns, Collection Tubes, User manual
Features:
- Yield: up to 15μg
- Ultra-fast procedure: 18 minipreps in 11min
- Elution volume: 50μL
- Sample material: 1mL to 3mL E. coli culture
- Very good price-performance ratio
- Silica-membrane technology
- Binding capacity: 15μg
- No use of organic solvents
- Ultra-fast small scale preparation of plasmid DNA
- Highly pure nucleic acids suitable for all common downstream applications
- Vectors <15kb
- Format: mini-spin columns
Tekniske data
Tekniske data
| Indhold og opbevaring | Room temperature (18–25°C) |
| Format | Mini Spin Column |
| Til brug med (udstyr) | Standard benchtop microcentrifuge |
| Til brug med (applikation) | Plasmid DNA purification/isolation |
| Inkluderer | Resuspension Buffer A1, Lysis Buffer A2 (without LyseControl), Neutralization Buffer A3, Wash Buffer AQ (Concentrate), Elution Buffer AE, RNase A (lyophilized), NucleoSpin™ Plasmid QuickPure Columns (dark yellow rings), Collection Tubes (2mL), User manual |
| Antal reaktioner | 50 Preps |
| Produkttype | NucleoSpin Plasmid QuickPure |
| RNase | Lyophilized |
| Prøvevolumen | Culture volume: 1–3mL high copy |
| Størrelse | <15 kbp |
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Sikkerhed og håndtering
- NucleoSpin Plasmid QuickPure
- Fare
- Akut toksicitet Kategori 4
- Hudætsning/hudirritation Kategori 2
- Alvorlige øjenskader/øjenirritation Kategori 2
- Respiratorisk sensibiliserende stof Kategori 1
- H302-Farlig ved indtagelse.
- H315-Forårsager hudirritation.
- H319-Forårsager alvorlig øjenirritation.
- H334-Kan forårsage allergi- eller astmasymptomer eller åndedrætsbesvær ved indånding.
- P261-Undgå indånding af pulver/røg/gas/tåge/damp/spray.
- P342+P311-Ved luftvejssymptomer: Ring til en GIFTINFORMATION/læge/ .
- MIXTURE LIST-Indeholder: sodium hydroxide solution (diluted < 2 %), dodecyl sulfate,sodium salt
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