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  5. Macherey-Nagel™ NucleoSpin™ miRNA, Mini kit for miRNA and RNA purification

Macherey-Nagel™ NucleoSpin™ miRNA, Mini kit for miRNA and RNA purification

Artikelnummer. 12788622
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250 reaktioner
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10 reaktioner
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50 reaktioner
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Artikelnummer. 12788622 Leverandør Macherey-Nagel™ Leverandørnr. 740971.250

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Beskrivelse

Isolate small and large RNA in parallel. NucleoSpin™ miRNA Kits work on a wide varitey of tissue and cell culture formats.

Features

  • Parallel isolation of small and large RNA
  • RNA purification fractionated by size:
  • Isolation of small RNA only (< 200 b),
  • Isolation of small RNA (< 200 b) and large RNA (> 200 b) in two separate fractions
  • Isolation of total RNA (small and large RNA in one fraction)
  • Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
  • Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform (patent pending)
  • NucleoSpin™ Filters for efficient sample homogenization
  • rDNase for efficient on-column removal of genomic DNA

Applications

  • Parallel isolation of small and large RNA from human / animal tissue and cultured cells, from plant tissue, and in combination with phenol / chloroform (e.g., TRIzol™) lysis
  • Purification of siRNA and large dsRNA from DICER reactions
  • Typical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization

Tekniske data

Format Mini spinsøjle
Til brug med (applikation) Purification of siRNA and large dsRNA from DICER reactions
Inkluderer NucleoSpin™ RNA Columns, NucleoSpin™ miRNA Columns, NucleoSpin™ Protein Removal Columns, Collection Tubes (2 mL, 2 mL lid, 1.5 mL), NucleoSpin™ Filters, buffers, RNase-free rDNase
Antal reaktioner 250 reaktioner
RNase Yes
Prøvestørrelse Small RNA: < 200 nt, large RNA: > 200 nt
Test tid <45 min./6 Reaction and <35 min./6 Reaction
Udbytte 10μg small RNA, 95μg large RNA
Produkttitel
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