missing translation for 'onlineSavingsMsg'
Få mere at vide
  1. Home
  2. Products
  3. Molekylærbiologiske reagenser og kits
  4. DNA-ekstraktion og oprensning
  5. Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up, kit for gel extraction or PCR clean up

Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up, kit for gel extraction or PCR clean up

Artikelnummer. 15116456
Skift visning
Klik for at se tilgængelige muligheder
Antal reaktioner:
240 forberedelser
50 forberedelser
Pakningsstørrelse:
240 forberedelser
50 reaktioner
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Artikelnummer. Antal reaktioner unitSize
15116456 240 forberedelser 240 forberedelser
12303368 50 forberedelser 50 reaktioner
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Denne vare kan ikke returneres. Se returpolicy
Artikelnummer. 15116456 Leverandør Macherey-Nagel™ Leverandørnr. 740609.240C

Venligst for at købe denne vare. Brug for en webkonto? Register dig hos os i dag!

Denne vare kan ikke returneres. Se returpolicy

Beskrivelse

Clean up PCR reactions and gel extractions with a two-in-one approach. Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit is optimized for recovery and elution volume when purifying DNA fragments via both applications.

NucleoSpin™ Gel and PCR Clean-up is designed for fast purification of PCR products, such as DNA from enzymatic reactions, as well as the extraction of DNA fragments from TAE or TBE agarose gels.

  • Two applications in one kit—One buffer to process both sample types, with only one loading step
  • High recovery for fragments down to 50 bp—Average recovery from 60–90%
  • Minimized elution volume of 15μL—Highly concentrated DNA
  • Binding buffer with pH indicator—Ensures optimal binding conditions
  • Separate buffers for single stranded DNA and RNA or for SDS-containing samples available separately
  • Suitable for all kinds of agarose gels and a variety of buffer systems (e.g. TAE, TBE)
  • Complete removal of contaminations: nucleotides, primers, enzymes, mineral oil, PCR additives, detergents, dyes
  • Applications: purification of PCR products, extraction of DNA from agarose or polyacrylamide gels, and downstream applications (cloning, sequencing, PCR, restriction analysis)

Kit contents: NucleoSpin™ Gel and PCR Clean-up columns, collection tubes (2mL), binding buffer with pH indicator, wash buffer and elution buffer

Tekniske data

Farve pH Indicator:
Yellow = correct conditions
Green = pH slightly high (adjustment recommended)
Blue = pH too high (adjustment necessary)
Format Mini spinsøjle
Isoleringsteknologi Silica membranteknologi
Effektivitet Typical recovery: 70–95%
Elueringsvolumen Elution volume: 30–100μL
Til brug med (udstyr) QIAcube™ compatible
TAE or TBE agarose gels, PCR
Inkluderer Binding Buffer NTI (2 x 75mL), Wash Buffer NT3 Concentrate (60mL), Elution Buffer NE (125mL), NucleoSpin Gel and PCR Clean-up Columns (4 x 60)
Antal reaktioner 240 forberedelser
Mængde 1 sæt
Renhed A260/A280: 1.8–1.9
Prøvevolumen <400 μL PCR reaction mixture, <400 μg agarose gel
Størrelse 50 bp to ∼20 kbp
Test tid 10 min/6 preps
Udbytte Up to ∼15μg DNA
Vis mere Vis mindre

Sikkerhed og håndtering

compliance-icons
Produktidentifikator
  • NucleoSpin Gel and PCR Clean-up
 Signalord
  • Advarsel
 Farekategori
  • Akut toksicitet Kategori 4
  • LANGTIDSFARE FOR VANDMILJØET Kronisk kategori 3
 Faresætning
  • H302-Farlig ved indtagelse.
  • H412-Skadelig for vandlevende organismer, med langvarige virkninger.
 Supplerende oplysninger
  • MIXTURE LIST-Indeholder: guanidinium thiocyanate
Produkttitel
Vælg et problem

Ved at klikke på Send, anerkender du, at du kan blive kontaktet af Fisher Scientific med hensyn til den feedback, du har givet i denne formular. Vi deler ikke dine oplysninger til andre formål. Alle angivne kontaktoplysninger skal også vedligeholdes i overensstemmelse med vores Privatlivspolitik.