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  5. Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up, kit for gel extraction or PCR clean up

Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up, kit for gel extraction or PCR clean up

Artikelnummer. 12303368
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Antal reaktioner:
240 forberedelser
50 forberedelser
Pakningsstørrelse:
240 forberedelser
50 reaktioner
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Artikelnummer. 12303368 Leverandør Macherey-Nagel™ Leverandørnr. 740609.50

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Beskrivelse

Clean up PCR reactions and gel extractions with a two-in-one approach. Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit is optimized for recovery and elution volume when purifying DNA fragments via both applications.

NucleoSpin™ Gel and PCR Clean-up is designed for fast purification of PCR products, such as DNA from enzymatic reactions, as well as the extraction of DNA fragments from TAE or TBE agarose gels.

  • Two applications in one kit—One buffer to process both sample types, with only one loading step
  • High recovery for fragments down to 50 bp—Average recovery from 60–90%
  • Minimized elution volume of 15μL—Highly concentrated DNA
  • Binding buffer with pH indicator—Ensures optimal binding conditions
  • Separate buffers for single stranded DNA and RNA or for SDS-containing samples available separately
  • Suitable for all kinds of agarose gels and a variety of buffer systems (e.g. TAE, TBE)
  • Complete removal of contaminations: nucleotides, primers, enzymes, mineral oil, PCR additives, detergents, dyes
  • Applications: purification of PCR products, extraction of DNA from agarose or polyacrylamide gels, and downstream applications (cloning, sequencing, PCR, restriction analysis)

Kit contents: NucleoSpin™ Gel and PCR Clean-up columns, collection tubes (2mL), binding buffer with pH indicator, wash buffer and elution buffer

Tekniske data

Farve pH Indicator:
Yellow = correct conditions
Green = pH slightly high (adjustment recommended)
Blue = pH too high (adjustment necessary)
Format Mini Spin Column
Isoleringsteknologi Silica Membrane Technology
Effektivitet Typical recovery: 70–95%
Elueringsvolumen Elution volume: 15–30μL
Til brug med (udstyr) TAE or TBE agarose gels, PCR
Inkluderer Binding Buffer NTI (40mL), Wash Buffer NT3 Concentrate (25mL), Elution Buffer NE (13mL), NucleoSpin Gel and PCR Clean-up Columns (50), 2mL Collection Tubes (50)
Antal reaktioner 50 forberedelser
Mængde 1 Kit
Renhed A260/A280: 1.8–1.9
Prøvevolumen <200 μL PCR reaction mixture, <200 mg agarose gel
Størrelse 50 bp to ∼20 kbp
Test tid 10 min/6 preps
Udbytte Up to ∼15μg DNA
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Sikkerhed og håndtering

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Produktidentifikator
  • NucleoSpin Gel and PCR Clean-up
 Signalord
  • Advarsel
 Farekategori
  • Akut toksicitet Kategori 4
  • LANGTIDSFARE FOR VANDMILJØET Kronisk kategori 3
 Faresætning
  • H302-Farlig ved indtagelse.
  • H412-Skadelig for vandlevende organismer, med langvarige virkninger.
 Supplerende oplysninger
  • MIXTURE LIST-Indeholder: guanidinium thiocyanate
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