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  5. Macherey-Nagel™ NucleoSpin™ 96 RNA, 96-well kit for RNA purification

Macherey-Nagel™ NucleoSpin™ 96 RNA, 96-well kit for RNA purification

Artikelnummer. 12768542
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Antal reaktioner:
2 x 96
24 x 96
4 x 96
Pakningsstørrelse:
192 reaktioner
2304 reaktioner
384 reaktioner
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12768542 24 x 96 2304 reaktioner
12736543 2 x 96 192 reaktioner
12756543 4 x 96 384 reaktioner
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Artikelnummer. 12768542 Leverandør Macherey-Nagel™ Leverandørnr. 740709.24

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Beskrivelse

Designed for manual or automated high throughput isolation of RNA from animal or human tissue. Macherey-Nagel™ NucleoSpin™ 96 RNA Kit enables time-saving parallel isolation of total RNA. The kit supplies all core content and convenient accessories for the highest efficiency.

NucleoSpin™ 96 RNA kits enable the isolation of RNA from up to 96 samples of fresh, frozen or stabilized animal or human cell cultures. NucleoSpin 96 RNA delivers consistent RNA quality, reproducible yields of RNA, and high-throughput procedures free of cross-contamination. The kits combine the stringency of guanidine-isothiocyanate lysis with effective digestion of genomic DNA by DNase I treatment and the speed of silica-membrane technology.

  • Time-saving parallel isolation of RNA from cultured cells or from saliva samples collected with Oragene™ RNA.
  • Suitable for manual processing under vacuum or by centrifugation, or used fully automated on common laboratory workstations.
  • Includes rDNase for efficient removal of genomic DNA and innovative MN Wash Plate to minimize the risk of cross-contamination.
  • Produces the highest purity total RNA, suitable for direct use in any kind of enzymatic reaction, such as RT-PCR, TaqMan, Northern Blot or microarray analysis.

Kit components: NucleoSpin RNA Binding Plates, MN Wash Plates, Square-well Blocks, Round-well Blocks Low, Elution Plates U-bottom, Self-adhering Foil, RNase-free rDNase, RNase-free water and all buffers (Lysis RA1; Wash RA2, RA3 and RA4; Reaction for rDNase)

  • Sample material: <107 cultured cells (centrifugation), <30 mg tissue (centrifugation), saliva (collected with Oragene™)
  • Fragment size: >200 nt
  • Typical yield: 20 μg (2 x 106 HeLa cells), 20 μg (20 mg mouse liver)
  • A260/A280: 1.90–2.10
  • Typical RIN (RNA integrity number): >9 (cells), ≥7 (tissue)
  • RNA ratio: 28S/18S approx. 2.1
  • Typical concentration: 50–200ng/μL
  • Elution volume: 50–130 μL
  • Preparation time: 70 min./plate
  • Binding capacity: 100 μg

Tekniske data

Koncentration 50–200ng/μL
Format 96-brønds plade
Isoleringsteknologi Siliciummembran
Elueringsvolumen Elution volume: 50–130μL
Prøvetype <1 x 107 Cultured Cells, <30 mg Tissue, Saliva (Collected with Oragene™)
Til brug med (applikation) Manual or automated isolation of total RNA from cultured cells and tissue
Inkluderer NucleoSpin RNA Binding Plates (24), 1.5mL Collection Tubes (96), MN Wash Plates with 6 paper sheets (24), Square-well Blocks (36), Round-well Blocks Low with PE Foil (24), Elution Plates U-bottom with PE Foil (24), RNase-free rDNase (48 vials), Lysis Buffer RA1 (12 x 125mL), Wash Buffer RA2 (6 x 360mL), Wash Buffer RA3 Concentrate (12 x 100mL), Wash Buffer RA4 Concentrate (12 x 65mL), Reaction Buffer for rDNase (6 x 60mL), RNase-free Water (12 x 125mL)
Antal reaktioner 24 x 96
Produkttype NucleoSpin 96 RNA
Renhed A260/A280: 1.90–2.10
Mængde 1 sæt
Prøvestørrelse <2 x 106 cultured cells, <20 mg human/animal tissue
Test tid 70 min/plate
Udbytte 20μg (2 × 106 HeLa cells)
20μg (20mg mouse liver)
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Produktidentifikator
  • NucleoSpin 96 RNA
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 Farekategori
  • Brandfarlig væske Kategori 3
  • Akut toksicitet Kategori 4
  • Respiratorisk sensibiliserende stof Kategori 1
  • LANGTIDSFARE FOR VANDMILJØET Kronisk kategori 3
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  • H226-Brandfarlig væske og damp.
  • H302-Farlig ved indtagelse.
  • H334-Kan forårsage allergi- eller astmasymptomer eller åndedrætsbesvær ved indånding.
  • H412-Skadelig for vandlevende organismer, med langvarige virkninger.
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  • P210-Holdes væk fra varme, varme overflader, gnister, åben ild og andre antændelseskilder. Rygning forbudt.
  • P264-Vask med vand grundigt efter brug.
  • P273-Undgå udledning til miljøet.
  • P301+P312-I TILFÆLDE AF INDTAGELSE: I tilfælde af ubehag ring til en GIFTINFORMATIO / læge /
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  • P342+P311-Ved luftvejssymptomer: Ring til en GIFTINFORMATION/læge/ .
 Supplerende oplysninger
  • MIXTURE LIST-Contain: guanidinium thiocyanate/ethanol
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