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  6. Macherey-Nagel™ NucleoSpin™ 96 Plasmid Transfection-grade Core Kit, 96-well kit

Macherey-Nagel™ NucleoSpin™ 96 Plasmid Transfection-grade Core Kit, 96-well kit

Product Code. 15533636
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Antal prøver:
24 x 96
4 x 96
Produkttype:
Fuldt sæt
Kernesæt
Unit Size:
2304 reaktioner
384 reaktioner
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Product Code. Antal prøver Produkttype unitSize
15533636 4 x 96 Fuldt sæt 384 reaktioner
15553636 4 x 96 Kernesæt 384 reaktioner
15543636 24 x 96 Kernesæt 2304 reaktioner
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Product Code. 15533636 Supplier Macherey-Nagel™ Supplier No. 740491.4

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Description

A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Full and Core kits utilize a 96-well plate format for an efficient high-throughput process.

Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.

  • Purifies high-copy plasmids for transfection
  • Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
  • Rapid purification from E. coli via automatic, manual, vacuum or centrifugation processing
  • Ultra-fast procedure simplifies the work-flow
  • High-throughput — 96-well plates allow for easy automation and supports plasmid screening
  • Downstream applications: transfection of common cell lines (lipofection, electroporation)
  • Also suitable for standard molecular biology application, such as cloning and sequencing

Specifications

Bakterielle endotoksiner <50 EU/ng DNA
Indhold og opbevaring Room temperature (18 - 25 °C)
Format 96-brønds plade
Isoleringsteknologi Silica Membrane and Endotoxin-reduction Technology
Prøvetype Bacterial Culture (E. coli, High-copy Plasmids)
Effektivitet Binding capacity: 20 μg
Elueringsvolumen Elution Volume: 100 - 200 μL
Endelig produkttype Transfection-grade plasmid DNA
Til brug med (udstyr) Centrifuge, Vacuum manifold (e.g. NucleoVac 96)
Til brug med (applikation) Rapid purification of plasmids for transfection
Inkluderer Filter Plates, Binding Plates, MN Wash Plates, Culture Plates, Elution Plates, Buffers, RNase A
Antal prøver 4 x 96
Produkttype Fuldt sæt
Renhed A260/A280: 1.80 - 1.85
Prøvevolumen Up to 5 mL
Størrelse < 15 kbp
Test tid 45 min/plate
Udbytte 5 - 20 μg
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Safety and Handling

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Product Identifier
  • NucleoSpin 96 Plasmid T.-grade (4x96)
 Signal Word
  • Fare
 Hazard Category
  • Akut toksicitet Kategori 4
  • Alvorlige øjenskader/øjenirritation Kategori 2
  • Brandfarlig væske Kategori 3
  • Respiratorisk sensibiliserende stof Kategori 1
  • Hudætsning/hudirritation Kategori 2
  • Specifik målorgantoksicitet - enkelt eksponering Kategori 2
 Hazard Statement
  • H226-Brandfarlig væske og damp.
  • H302-Farlig ved indtagelse.
  • H315-Forårsager hudirritation.
  • H319-Forårsager alvorlig øjenirritation.
  • H334-Kan forårsage allergi- eller astmasymptomer eller åndedrætsbesvær ved indånding.
  • H336-Kan forårsage sløvhed eller svimmelhed.
 Precautionary Statement
  • P210-Holdes væk fra varme, varme overflader, gnister, åben ild og andre antændelseskilder. Rygning forbudt.
  • P260-Indånd ikke pulver/røg/gas/tåge/damp/spray.
  • P261-Undgå indånding af pulver/røg/gas/tåge/damp/spray.
  • P264-Vask grundigt efter brug.
  • P280-Bær beskyttelseshandsker/beskyttelsestøj/øjenbeskyttelse/ ansigtsbeskyttelse.
  • P301+P312-I TILFÆLDE AF INDTAGELSE: I tilfælde af ubehag ring til en GIFTINFORMATIO / læge /
  • P330-Skyl munden.
  • P342+P311-Ved luftvejssymptomer: Ring til en GIFTINFORMATION/læge/ .
 Supplemental information
  • MIXTURE LIST-Contain: sodium hydroxide, dodecyl sulphate, guanidine hydrochloride,triethanolamine, 2-propanol, RNAse
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