Macherey-Nagel™ NucleoSpin™ 8 Virus, 8-well kit for viral RNA/DNA purification

NucleoSpin 8 Virus enables rapid simultaneous preparation of viral nucleic acids from serum, plasma or any cell-free biological fluid. The kits allow reliable purification of viral RNA and DNA without cross-contamination. NucleoSpin 8 Virus is primarily designed for manual use in a centrifuge. NucleoSpin 8 Virus Core Kits allow for more variation and higher flexibility in the consumables used for lysis, washing and elution and are primarily recommended for manual or automated vacuum use. With the NucleoSpin 8 Virus method, RNA viruses are lysed quickly and efficiently by a lysis buffer which is a highly concentrated solution of GITC. DNA viruses (e.g. HBV) are usually more difficult to isolate and require a digestion of samples with proteinase K. Lysis buffer and ethanol create appropriate conditions for binding of nucleic acids to the silica membrane of the NucleoSpin virus binding strips or plate. Carrier RNA included in lysis buffer improves binding and recovery of the low concentrated viral RNA/DNA. Contaminations (potential PCR inhibitors) like salts, metabolites and soluble macromolecular cellular components are removed in washing steps with ethanolic wash buffers. The purified viral nucleic acids can be eluted in low salt buffer or water and are ready-to-use in subsequent downstream applications like RT-PCR or PCR.

Centrifugation

For centrifuge processing of NucleoSpin 8 Virus kits a microtiterplate centrifuge is required which is able to accommodate the NucleoSpin Virus Strips/Plate stacked on a round- or square-well block or rack with tube strips and reaches accelerations of 5,600 – 6,000 x g (bucket height: 85 mm).For centrifuge processing of the 8-well strips the reusable starter set C, containing column holders C, dummy strips, MN square-well blocks, tube strips is required.

Vacuum processing

The NucleoSpin 8 Virus kits can be used manually with the NucleoVac 96 vacuum manifold.For vacuum processing of the 8-well Strips the reusable Starter Set A, containing column holders A and dummy strips is required. For automation on laboratory platforms with standard 96-well plate vacuum chambers the use of the starter set A is also required.

Kit components: NucleoSpin Virus binding strips, MN square-well blocks, racks with tube strips, cap strips, self-adhering foil, buffers, carrier RNA, proteinase K.

• Elution volume: 70 to 100 μL
• Typical recovery: > 90%
• Binding capacity: 40 μg
• Preparation time: 60 min./6 strips