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Macherey-Nagel™ NucleoBond™ RNA Soil Mini kit for RNA from soil

Artikelnummer. 15858096
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Antal reaktioner:
2
10
50
Produkttype:
RNA Kit
RNA-kit + DNA-sæt
Pakningsstørrelse:
10 reaktioner
2 reaktioner
50 reaktioner
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Artikelnummer. Antal reaktioner Produkttype unitSize
15858096 10 RNA Kit 10 reaktioner
15868096 50 RNA Kit 50 reaktioner
15937282 2 RNA-kit + DNA-sæt 2 reaktioner
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Artikelnummer. 15858096 Leverandør Macherey-Nagel™ Leverandørnr. 740142.10

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Easy handling and superior speed in RNA purification from soil samples for qRT-PCR analysis, as well as parallel preparation of DNA. Complete kit uses anion exchange chromatography and gravity flow columns combined with NucleoSpin Bead Tubes Type A (included).

NucleoBond™ RNA Soil Mini kit enables efficient isolation of high quality RNA and DNA from any kind of soil or sediment sample. Most soils contain relatively low amounts of RNA, compared to DNA. Therefore, the kit is designed with proven NucleoBond™ technology to enable high sample input and and pure nucleic acids.

  • Anion exchange technology to optimize RNA yield and purity—suitable for qRT-PCR analysis
  • Combination of mechanical homogenization and chemical lysis allows processing of large samples
  • Parallel preparation of RNA and DNA in one hour*
  • Sample disruption is achieved with NucleoSpin™ Bead Tubes Type A, optimized Lysis Buffer, and Phenol:Chloroform:Isoamylalcohol
  • Optional Buffer OPT enables RNA and DNA purification from mineralic and organic soil samples

* DNA isolation requires the DNA Set for NucleoBond™ RNA Soil Mini, sold separately (man. part no. 740143.10 or 740143.50) or in a combo kit

Tekniske data

Format Gravity Flow Column
Isoleringsteknologi Anion Exchange Chromatography, Gravity Flow Columns, NucleoSpin Bead Tubes
Elueringsvolumen Elution volume: 50-100 μL
Prøvetype Jord
Effektivitet Binding capacity: 30 μg
Til brug med (udstyr) Centrifuge for microcentrifuge tubes
Til brug med (applikation) RNA/DNA isolation from soil for qRT-PCR
Inkluderer NucleoBond Soil Mini Columns, NucleoSpin Bead Tubes Type A, NucleoSpin RNA columns, Buffers
Antal reaktioner 10
Produkttype RNA Kit
Renhed A260/A280: 1.5 - 2.0
Prøvestørrelse 0.25 to 0.5 g
Test følsomhed RNA integrity number (RIN): > 7.0
Test tid 60 min/12 preps (RNA)
Udbytte 0.25 to 2.5 μg
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Produktidentifikator
  • NucleoBond RNA Soil Mini (10)
Signalord
  • Advarsel
Farekategori
  • Alvorlige øjenskader/øjenirritation Kategori 2
  • Brandfarlig væske Kategori 3
Faresætning
  • H226-Brandfarlig væske og damp.
  • H319-Forårsager alvorlig øjenirritation.
Supplerende oplysninger
  • MIXTURE LIST-Indeholder: EDTA, tris(hydroxymethyl)aminomethane, sodium chloride, dodecyl sulphate sodiumsalt, potassium chlo
Produkttitel
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