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Invitrogen™ Human TNF alfa ELISA Kit, Ultrasensitiv

Artikelnummer. 10465133
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Mængde:
2 x 96 prøver
5 x 96 prøver
96 prøver
Pakningsstørrelse:
192 tests
96 tests
Pakke med 5
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10465133 96 prøver 96 tests
10160573 2 x 96 prøver 192 tests
10475133 5 x 96 prøver Pakke med 5
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Artikelnummer. 10465133 Leverandør Invitrogen™ Leverandørnr. KHC3014
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Brug rabatkode 28170
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Forventet forsendelse: 06-08-2026
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6754.50 DKK valid until 2026-12-31
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Beskrivelse

Indeholder

Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

ELISA

Human Tumor Necrosis Factor Alpha (Hu TNFα ) ELISA kvantificerer Hu TNFα i humant serum, plasma, bufferopløsning eller cellekulturmedium. Assayet vil udelukkende genkende både naturlig og rekombinant Hu TNFα . Metodens princip The Human TNFα ultrafølsom solid-fase sandwich ELISA (enzym-linked immunosorbent assay) er designet til at måle mængden af målet bundet mellem et matchet antistofpar. Et målspecifikt antistof er blevet præcoatet i brøndene på den medfølgende mikroplade. Prøver, standarder eller kontroller tilsættes derefter til disse brønde og binder til det immobiliserede (fangende) antistof. Sandwichen dannes ved tilsætning af det andet (detektor) antistof, en substratopløsning tilsættes, der reagerer med enzym-antistof-målkomplekset for at producere et målbart signal. Intensiteten af dette signal er direkte proportional med koncentrationen af mål til stede i den originale prøve. Strenge validering Hvert fremstillet parti af dette ELISA kit er kvalitetstestet for kriterier som sensitivitet, specificitet, præcision og lot-to-lot konsistens. Se manualen for mere information om validering.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Ordreinfo

Forsendelsestilstand: Våd is

TRUSTED_SUSTAINABILITY

Tekniske data

Adgangsnummer P01375
Analyseområde 0,5 til 32 pg/ml
Assay følsomhed <0.09 pg/mL
Indhold og opbevaring Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Konjugeret HRP
Immunoassay-kitformat Sandwich ELISA Kit
Produkttype ELISA
Protein familie Cytokines & Receptors
Prøvetype Plasma, Serum, Supernatant
Målarter ADN
Til brug med (applikation) Kolorimetrisk
Til brug med (udstyr) Kolorimetrisk mikropladelæser
Gene Alias APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factorAPC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor; DIF-alpha; TNFA; TNFSF2; TNLG1F; TNF
Gen-id (Entrez) 7124
Gen symbol DIF-alfa,TNFA,TNFSF2,TNLG1F,TNF
Interassay CV 8.5%
Intraassay CV 5.9%
Sættets indhold Forbelagt 96-brønds plade, Standard, Standardfortyndingsbuffer, Biotinyleret detektionsantistof, Streptavidin-HRP, HRP fortynder, Vaskebuffer, kromogen, Stop Løsning, Klæbende pladedæksler
Etiket eller farvestof Biotin
Præcision <0.09 pg/mL
Mængde 96 prøver
Regulatorisk status RUO
Prøvevolumen Plasma 50μ L, Serum 50μ L, Supernatant 50μ L
Forsendelsestilstand Wet Ice
Opbevaringskrav 2 °C til 8 °C
Mål TNF alfa
Test tid 1 t 20 min
Samlet analysetid 3 hr
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