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Invitrogen™ Cytochrome C Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ KHO1051

3613.51 DKK valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.10000kg

Product Code. 10778395

  • 5420.00 DKK / Each
Estimated Shipment: 10-04-2024
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Includes: Cytochrome C Coated 96-Well Plate; Cytochrome C Total Standard; Standard Diluent Buffer; Cytochrome C Total Detection Antibody; anti-rabbit IgG-HRP (100X); HRP Diluent; Wash Buffer Concentrate (25X); Stabilized Chromogen, TMB; Stop Solution; Plate Covers; Detailed protocol with validation tests

Description

Description

The Human Cytochrome C ELISA quantitates Hu Cytochrome C in human cell and tissue lysates. Cross reactivity has been observed in mouse and rat cells. The assay will exclusively recognize both natural and recombinant Hu Cytochrome C. Principle of the method The Human Cytochrome C solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

Order Info

Shipping condition: Wet ice

Specifications

Specifications

P62897,P62898,P99999
<0.156 ng/mL
Biotin
Cell Lysates
Colorimetric Microplate Reader
CYC,HCS,THC4
6.7%
Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Cytochrome C
RUO
2°C to 8°C
1 hr. 20 min.
0.078-5 ng/mL
0.078 to 5ng/mL
ELISA Kit
Human, Mouse, Rat
13063, 25309, 54205
4 hr.
4.6%
HRP
96 Tests
Cell Lysate,10 μL
Human, Mouse, Rat
4 hr.
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For Research Use Only. Not for use in diagnostic procedures.