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Invitrogen™ IFN gamma Porcine ELISA Kit
Quantifies natural and recombinant swine IFN γ in serum, buffered solution, or cell culture medium
4945.00 DKK - 7570.00 DKK
Specifications
| Accession Number | P17803 |
|---|---|
| Assay Range | 7.8-500 pg/mL |
| Assay Sensitivity | <2 pg/mL |
| Conjugate | Biotin |
| Product Type | ELISA Kit |
Includes: Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.
Description
The Porcine Interferon gamma (Porcine IFN-γ) ELISA quantitates Porcine IFN-gamma in serum, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Porcine IFNγ. Principle of the method The Porcine IFNγ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
IFN γ is a lymphokine produced by activated T and NK cells.
Cell Analysis, Cell Signaling, Cell Viability, Proliferation & Function, Immunoassays, Ready-To-Use Immunoassay
Order Info
Shipping Condition: Wet Ice
Specifications
| P17803 | |
| <2 pg/mL | |
| ELISA Kit | |
| Porcine | |
| Colorimetric Microplate Reader | |
| IFNG | |
| 7.8% | |
| Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| IFN-γ | |
| Serum, 50 μL; Supernatant, 50 μL | |
| Porcine | |
| 3 hr. |
| 7.8-500 pg/mL | |
| Biotin | |
| Serum, Supernatant | |
| Colorimetric Detection | |
| 396991 | |
| 3 hr. | |
| 4.2% | |
| HRP | |
| RUO | |
| 2°C to 8°C | |
| 1 hr. 20 min. |
For Research Use Only. Not for use in diagnostic procedures.