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Invitrogen™ IL-8 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ KAC1301

Additional Details : Weight : 0.35000kg Transport : UN number : 3316 Chem class : 9 Pack group : III

Product Code. 10008992

  • 4945.00 DKK / 96 tests
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Includes: Pre-coated plate, standard, controls, antibody-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

Description

The Human Interleukin 8 (Hu IL-8) ELISA quantitates Hu IL-8 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-8. Principle of the method The HumanIL-8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-8 (Interleukin-8) is a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.

Order Info

Shipping Condition: Wet ice

Specifications

Specifications

P10145
0.7 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1
4.2%
HRP
96 Tests
Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
Human
2 hr. 30 min.
7-750 pg/mL
HRP
Plasma, Serum, Supernatant
Colorimetric
3576
3.7%
Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls
0.7pg/mL
RUO
2°C to 8°C
1 hr. 20 min.
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For Research Use Only. Not for use in diagnostic procedures.