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BD Biosciences™ NKTEST™ (CE/IVD)

Artikelnummer. 15991230
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Oversigt:
100 tests
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15991230 100 tests
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Artikelnummer. 15991230 Leverandør BD Biosciences™ Leverandørnr. 659179

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Beskrivelse

For quantitative determination of the cytotoxic activity of human natural killer (NK) cells.

  • This reagent kit allows the quantitative determination of the cytotoxic activity of human natural killer (NK) cells.
  • Contains cryopreserved prestained K562 target cells, complete medium and a DNA-staining reagent.
  • The K562 target cells are labelled with a lipohilic green fluorescent membrane dye in order to discriminate effector and target cells.
  • After the incubation period in the cytotoxicity assay, killed target cells are identified by a DNA-stain, which penetrates the dead cells and specifically stains their nuclei.
  • In this manner, the percentage of target cells killed by effector NK cells can be determined.
TRUSTED_SUSTAINABILITY

Tekniske data

Indhold og opbevaring Reagent A (complete medium) and reagent C (DNA staining solution) must be stored at 2-8°C (in refrigerator). Store Reagent B (Interleukin-2) at >-15°C. The K562 target cells have to be stored at - 70°C
Oversigt 100 tests
Detektionsmetode Flow Cytometry, Stain
Mængde 5 x 100 mL bottles Complete Medium, 5 x 300 μL vials Interleukin-2 (200 U/mL), 5 mL DNA Staining Solution, 5 vials of cryopreserved, prestained K562 Target Cells
Stabilitet Cells are stable for 12 weeks showing a viability >90%.
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