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NK1.1, Super Bright 600, clone: PK136, eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen 63-5941-80

Additional Details : Weight : 0.01000kg

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Product Code. 15855613

  • 1056.00 DKK / 25µg


Estimated Shipment date
from Supplier 20-12-2018
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Description

Description

Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. No. 14-5971) should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.Applications Reported: This PK136 antibody has been reported for use in flow cytometric analysis.Applications Tested: This PK136 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser
Specifications

Specifications

NK1.1
Monoclonal
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P27814
CD161, NKR-P1C, Ly-55
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
PK136
Super Bright 600
Klrb1c
Liquid
KLRB1C
IgG2a, kappa
25μg
4°C, store in dark, DO NOT FREEZE!
Primary
17059
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