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NIH/3T3 (mouse NIH/Swiss embryo) Whole cell lysate, Denatured; Abnova

Artikelnummer. 16079545
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Mængde:
200 μg
Pakningsstørrelse:
200µg
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16079545 200 μg 200µg
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Artikelnummer. 16079545 Leverandør Abnova Leverandørnr. L018V1.200ug

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Beskrivelse

Non-denatured whole cell lysate of NIH/3T3 (mouse NIH/Swiss embryo)

  • Tissue: Embryo, Contact-Inhibited
  • Host: Mouse
  • Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
  • Storage buffer: In ready-to-use 1X Sample Buffer (50mM Tris-HCl, 2% SDS, 10% glycerol, 300mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Western Blotting

Tekniske data

Til brug med (applikation) Western Blot
Væv Embryo, Contact-inhibited
Oversigt Whole cell lysate (denatured)
Lyseringsbuffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Fremstillingsmetode Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Kvalitetskontrol test 12.5% SDS-PAGE Stained with Coomassie Blue.
Mængde 200 μg
Opbevaringsbuffer In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Værtsarter Mouse
Indhold og opbevaring Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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