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Cytiva Ni Sepharose™ excel

Product Code. p-4518347 Shop All Cytiva Products
25 mL, 25mL
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Quantity:
25 mL
100 mL
500 mL
Unit Size:
100mL
25mL
500mL
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity.
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling.
  • Choose between several formats for screening and preparative purification of histidine-tagged proteins.
  • Ni Sepharose excel is an IMAC medium precharged with nickel ions very strongly bound to a chelating ligand, allowing loading of samples that usually cause stripping of metal ions.
  • Ni Sepharose excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.

TRUSTED_SUSTAINABILITY

Specifications

Buffer 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
Product Type Ni Sepharose excel chromato graphy media
Quantity 25 mL
Flow Rate 150 to 600cm/hr.
pH Range 2 to 12
Particle Size 90ÎĽm
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4° to 30°C
For Use With (Application) Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromatography (IMAC)
Matrix Ni-Sepharose

Safety and Handling

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Signal Word
  • ||Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • EUH208-Contains Nickel. May produce an allergic reaction.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : ethanol
  • UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.
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