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Invitrogen™ MultiShot™ BL21(DE3) Chemically Competent E. coli Cells, 96-well plate

Artikelnummer. 31781154
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1 x 96 Well Plate (20 μL/Well)
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31781154 1 x 96 Well Plate (20 μL/Well)
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Artikelnummer. 31781154 Leverandør Invitrogen™ Leverandørnr. A40007051

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Beskrivelse

MultiShot BL21(DE3) Chemically Competent E. coli Cells enable high-throughput protein expression with compatibility for automated liquid handling. This ready-to-use format streamlines cloning and expression workflows using the reliable BL21(DE3) expression strain.

Invitrogen MultiShot BL21(DE3) Chemically Competent E. coli Cells are suitable for the expression of non-toxic heterologous genes. They are pre-aliquoted into a 96-well PCR plate to increase transformation productivity.

Features of MultiShot BL21(DE3) competent cells include:

  • High transformation efficiency: >1x107 cfu/μg pUC19 DNA
  • Specifically constructed for high-level expression of recombinant proteins
  • Microtiter plate format design convenient for automated high-throughput cloning
  • Competent cells pre-aliquoted into 96-well plate, ready for transformation
  • S.O.C. Outgrowth medium included

MultiShot BL21(DE3) competent cells in a 96-well plate are designed to facilitate high-throughput transformation compatible with liquid handling equipment. The BL21 (DE3) strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG. BL21(DE3) is an E. coli B strain and does not contain the lon protease. It is also deficient in the outer membrane protease OmpT. The lack of these two key proteases reduces degradation of heterologous proteins expressed in the cells.

Genotype

F– ompT hsdSB (rB–, mB–) gal dcm (DE3)

TRUSTED_SUSTAINABILITY

Tekniske data

Produkttype Chemically competent cells
Inkluderer pUC19 DNA, S.O.C media
Transformationseffektivitetsniveau ≥1.0 x 107
Forsendelsestilstand Dry Ice
Arter E. coli
Format 96-well Plate
Produktlinje MultiShot
Til brug med (applikation) Protein Expression
Mængde 1 x 96 Well Plate (20 μL/Well)

For Research Use Only.

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