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MP Biomedicals™ Trypsin, Porcine Pancreas

Typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

Brand:  MP Biomedicals™ 0215357125

Additional Details : CAS Number : 9002-07-7 Weight : 0.02500kg


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Product Code. 11492922

  • 563.00 DKK / 25g


Estimated Shipment
28-07-2021
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Description

Description

  • CAS Number: 9002-07-7
  • Molecular Weight: Approximately 23,000 or 22000 (for human)
  • Composition: Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain.
  • Activity: One BAEE unit will produce a DA253 of 0.001 per minute at pH 7.6 at 25°C using BAEE as substrate. Reaction volume = 3.2mL (1 cm light path).
    One TAME unit hydrolyzes 1 umole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25°C, pH 8.2, in the presence of 0.001 M calcium ion.
    One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified.
  • Activity Conversion:1 TAME unit = 19.2 USP or NF units = 57.5 BAEE Units
  • Composition: Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain.
Specifications

Specifications

Powder
Trypsin
Porcine
Release adherent cells from tissue culture plates for passaging
25g
4°C
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Safety and Handling

Safety and Handling

Signal Word
  • Danger

Hazard Category
  • Respiratory sensitiser Category 1
  • Serious eye damage/eye irritation Category 2
  • Specific target organ toxicity Category 3
  • Skin corrosion/irritation Category 2
  • Skin sensitiser Category 1

Hazard Statement
  • H315-Causes skin irritation.
  • H317-May cause an allergic skin reaction.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H335-May cause respiratory irritation.

Precautionary Statement
  • P101-If medical advice is needed, have product container or label at hand.
  • P102-Keep out of reach of children.
  • P103-Read label before use.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P284-¢In case of inadequate ventilation! wear respiratory protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P321-Specific treatment (see on this label).
  • P405-Store locked up.
  • P501-Dispose of contents/container to in accordance with local/regional/national/international regulations.

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