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  5. Invitrogen™ Mus TGF beta-1 ELISA Kit

Invitrogen™ Mus TGF beta-1 ELISA Kit

Artikelnummer. 15512027
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Mængde:
10 x 96 tests
2 x 96 prøver
5 x 96 prøver
96 prøver
Pakningsstørrelse:
96 tests
Pakke med 10
Pakke med 2
Pakke med 5
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Artikelnummer. 15512027

Brand: Invitrogen™ BMS6084

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3936.80 DKK valid until 2025-12-31
BEDSTE tilbudspris! Brug kampagnekoden "24080" for at få din tilbudspris.
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Beskrivelse

Indeholder

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse TGF-beta1
Biotin-Conjugate anti-mouse TGF-beta1 polyclonal antibody
Streptavidin-HRP
Mouse TGF-beta1 Standard lyophilized, 4ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
]Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

ELISA

The Mouse Transforming Growth Factor beta 1 (βMs TGF 1) ELISA βkvantificerer Ms TGF 1 i museserum, plasma, bufferopløsning eller cellekulturmedium. Assayet vil udelukkende genkende både naturlig og rekombinant muse-TGF β1. Metodens princip Musen TGF 1 βsolid-fase sandwich ELISA (enzym-linked immunosorbent assay) er designet til at måle mængden af målet bundet mellem et matchet antistofpar. Et målspecifikt antistof er blevet præcoatet i brøndene på den medfølgende mikroplade. Prøver, standarder eller kontroller tilsættes derefter til disse brønde og binder til det immobiliserede (fangende) antistof. Sandwichen dannes ved tilsætning af det andet (detektor) antistof, en substratopløsning tilsættes, der reagerer med enzym-antistof-målkomplekset for at producere et målbart signal. Intensiteten af dette signal er direkte proportional med koncentrationen af mål til stede i den originale prøve. Strenge validering Hvert fremstillet parti af dette ELISA kit er kvalitetstestet for kriterier som sensitivitet, specificitet, præcision og lot-to-lot konsistens. Se manualen for mere information om validering.

TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.
TRUSTED_SUSTAINABILITY

Tekniske data

Adgangsnummer P04202
Analyseområde 31,25 til 2.000 pg/ml
Assay følsomhed 7.8 pg/mL
Konjugeret HRP
Immunoassay-kitformat Sandwich ELISA Kit
Produkttype ELISA
Prøvetype Plasma, Serum, Supernatant
Målarter Mus
Til brug med (applikation) ELISA
Til brug med (udstyr) Kolorimetrisk mikropladelæser
Gene Alias CED; cytokine; DPD1; LAP; Latency-associated peptide; prepro-transforming growth factor beta-1; regulatory protein; TGF b; TGF b 1; TGF beta; tgf beta 1; tgf beta1; TGF beta-1; TGF beta-1 protein; TGF β; TGF β 1; TGF β1; Tgfb; TGFB1; TGF-B1; Tgfb-1; TGFbeta; TGF-beta 1; TGF-beta 1 protein; TGFbeta1; TGF-beta1; TGF-beta-1; TGFβ1; Transforming growth factor; transforming growth factor b1; transforming growth factor beta; transforming growth factor beta 1; transforming growth factor beta-1; Transforming growth factor beta-1 proprotein; transforming growth factor beta-1 proprotein; transforming growth factor beta-1; transforming growth factor β1; transforming growth factor, beta 1; transforming growth factor, beta 1 (Camurati-Engelmann disease); transforming growth factor, beta-1; transforming growth factor-beta 1; transforming growth factor-beta I; transforming growth factor-beta-1; transforming growth factor-beta-1 precursor; unnamed protein productCED; cytokine; DPD1; LAP; Latency-associated peptide; prepro-transforming growth factor beta-1; regulatory protein; TGF b; TGF b 1; TGF beta; tgf beta 1; tgf beta1; TGF beta-1; TGF beta-1 protein; TGF β; TGF β 1; TGF β1; Tgfb; TGFB1; TGF-B1; Tgfb-1; TGFbeta; TGF-beta 1; TGF-beta 1 protein; TGFbeta1; TGF-beta1; TGF-beta-1; TGFβ1; Transforming growth factor; transforming growth factor b1; transforming growth factor beta; transforming growth factor beta 1; transforming growth factor beta-1; Transforming growth factor beta-1 proprotein; transforming growth factor beta-1 proprotein; transforming growth factor beta-1; transforming growth factor β1; transforming growth factor, beta 1; transforming growth factor, beta 1 (Camurati-Engelmann disease); transforming growth factor, beta-1; transforming growth factor-beta 1; transforming growth factor-beta I; transforming growth factor-beta-1; transforming growth factor-beta-1 precursor; unnamed protein product; TGF-beta1; TGFbeta1; Tgfb; Tgfb-1
Gen-id (Entrez) 21803
Gen symbol TGF-beta1,TGFbeta1,Tgfb,Tgfb-1
Interassay CV 5.8%
Intraassay CV 7.9%
Sættets indhold Forbelagt 96-brønds plade, Standard, Prøve fortynder, Analysebufferkoncentrat, HRP-konjugeret detektionsantistof, Vaskebuffer, kromogen, Stop Løsning, Klæbende pladedæksler
Etiket eller farvestof Biotin
Protein aliaser TGF-beta-1, LAP
Mængde 96 prøver
Regulatorisk status RUO
Prøvevolumen Plasma 20μ L, Serum 20μ L, Supernatant 20μ L
Opbevaringskrav 2 °C til 8 °C
Synonym Camurati-Engelmanns sygdom, DPD1
Mål TGF beta-1
Test tid 1 t 20 min
Samlet analysetid 4 hr
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