Invitrogen™ MCPT-1 (mMCP-1) Mouse Uncoated ELISA Kit with Plates
Optimized for the accurate and precise measurement of mouse MCPT-1 in serum, and tissue culture supernatant samples. MCPT-1 (Mast Cell Protease-1) is a β-chymase, a type of serine protease stored and secreted in a tissue-specific manner by mucosal mast cells.
Brand: Invitrogen™ 88-7503-22
Additional Details : Weight : 0.10000kg
DescriptionMCPT-1 is the only chymase expressed by intestinal mucosal mast cells, which are found in the intestinal epithelium. Although it is expressed constitutively and is detectable in the sera of normal mice, parasites in the gut cause systemic levels to increase dramatically within two days and peak at two weeks following infection. It plays an important role in host defense against intestinal parasites, as mice deficient in MCPT-1 display delayed response upon infection. Elevated MCPT-1 levels are also observed during intestinal allergic hypersensitivity reactions. The mechanism of its action is not fully understood, although it is believed to increase intestinal permeability similarly to rMCPT-II, possibly by cleaving the proteins at tight junctions between cells.
|2°C to 8°C|
|24 hr. 30 min.|
|Mouse Mast Cell Protease-1|
|Colorimetric Microplate Reader|
|Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for the dilution of antibodies and standards, 96-Well Plates: Corning Costar flat-bottom plated (included with catalog numbers ending in -22 and -86)|
|Serum, 100 μL|Supernatant, 100 μL|
|1 hr. 45 min.|
|2 x 96 Tests|
For Research Use Only