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Proteintech™ Mouse IL-15 ELISA Kit
KE10028 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL15 ELISA kit is to be used to detect and quantify protein levels of endogenous IL15.
Brand: Proteintech™ KE10028-96T
This item is not returnable.
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Description
- The assay recognizes mouse IL15. An antibody specific for IL15 has been pre-coated onto the microwells. The IL15 protein in samples is captured by the coated antibody after incubation.
- Following extensive washing, another antibody of biotinylated specific for IL15 is added to detect the captured IL15 protein.
- For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent.
- Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
- Interleukin 15 (Il15) is a cytokine important for the development, maturation, and function of many cells of the immune system including NK, NKT, gammadeltaT, and CD8(+) T cells.
- Its expression affects homeostasis and function of B cells through NK cell-derived IFNG. Il-15 binds a trimeric Il-15 receptor (Il-15R) complex formed by a specific receptor chain (Il-15Rα) combined with theβandγchains of the IL-2R, acting as signal transducing components.
- The transgenic mice overexpressing Il15 exhibit an increase in the number of memory CD8+ T cells in a naive state and enhanced protection against bacterial infections. Mice lacking Il15 exhibit impaired protection against a strain of attenuated Mycobacterium
Specifications
| NP_032383 | |
| 50.2 pg/mL | |
| Sandwich ELISA, Double Antibody | |
| Plasma, Serum | |
| Mouse | |
| Mouse | |
| <10% | |
| Blue ice | |
| 4.5 hr. |
| 250 to 8000 pg/mL | |
| ISO 13485 | |
| Coated ELISA Kit | |
| DAB | |
| 16168 | |
| Solid | |
| <10% | |
| -20°C to +4°C | |
| P48346 |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- 96TEST Mouse IL-15 ELISA Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P234-Keep only in original container.
- P264-Wash skin thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P362-Take off contaminated clothing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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Product Title
Proteintech™ Mouse IL-15 ELISA Kit > Target Species: Mouse
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