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Invitrogen™ IGF-2 Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMIGF2

2983.48 DKK valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.00400kg

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Product Code. 15445283

  • 4475.00 DKK / 1 set
Estimated Shipment: 11-04-2024
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Description

Description

The Mouse Insulin-like Growth Factor 2 (Ms IGF-2) ELISA quantitates Ms IGF-2 in mouse cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IGF-2. Principle of the method The Mouse IGF-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The insulin gene family, comprised of insulin, relaxin and insulin-like growth factors I and II (IGF-I and IGF-II), represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somato-medins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth. IGF-I and -II are critical regulators of cell proliferation and differentiation. Most of the growth promoting properties of both ligands are mediated by the IGF-I receptor (IGF-IR). IGF- I and -II, respectively known as somatomedin C and somatomedin A, are single chain polypeptides which share an amino acid sequence homology of about 47% with insulin. IGF-I expression is regulated by growth hormone and mediates postnatal growth, while IGF-II is induced by placental lactogen during prenatal development. IGF-II is a fetal growth factor, influenced by placental lactogen and abundantly expressed by placental trophoblasts. IGF-II and IGF-binding protein 1 (IGFBP1) gene variants are associated with overfeeding-induced metabolic changes. The human IGF-II gene maps to chromosome 11p15.5, encoding a 180 amino acid protein which is the precursor to IGF-II.
Specifications

Specifications

P09535
1500 pg/mL
Solid-phase sandwich ELISA
ELISA Kit
Supernatant
96 assays
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
16002
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Development
2°C to 8°C
Mouse
4 hr. 45 min.
2.3-150 ng/mL
1500pg/mL to 150ng/mL
Biotin
Growth Factors & Receptors
The antibody pair provided in this kit recognizes mouse IGF-II (for Cell Culture).
Mouse
Colorimetric Microplate Reader
AL033362,Igf-2,Igf-II,M6pr,Mpr,Peg2
<10%
HRP
RUO
Supernatant, 100 μL
AL033362, Igf-2, Igf-II, M6pr, Mpr, Peg2
1 hr. 20 min.
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