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Invitrogen™ HGF Mouse ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EMHGF
Additional Details : Weight : 0.00400kg
Description
The Mouse Hepatocyte Growth Factor(Ms HGF) ELISA quantitates Ms HGF in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms HGF. Principle of the method The Mouse HGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Hepatocyte growth factor (HGF) is a member of the plasminogen subfamily of S1 peptidases. The inactive polypeptide formed upon translation undergoes cleavage to produce a 69 kDa alpha-chain and 34 kDa beta-chain. A disulphide bond between the two chains makes active HGF. HGF is secreted by mesenchymal cells and stimulates mitogenesis, cell motility, and matrix invasion. It is involved in cellular growth and morphogenesis in a wide variety of tissue types. In humans, HGF is present on chromosome 7.Specifications
| Q08048 | |
| 400 pg/mL | |
| HGF ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Chemokines and Receptors Protein Family, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of HGF in Samples | |
| Solid-phase sandwich ELISA | |
| Colorimetric | |
| ELISA Kit | |
| Plasma, Serum, Supernatant | |
| 96 Assays | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 15234 | |
| Mouse | |
| <10% | |
| HRP | |
| RUO | |
| Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL | |
| C230052L06Rik, HGF/SF, NK1, NK2, SF, SF/HGF | |
| 1 hr. 20 min. |
| 0.614-60 ng/mL | |
| 400pg/mL to 60ng/mL | |
| HGF | |
| Biotin | |
| Sandwich ELISA Kit | |
| Chemokine & Receptors | |
| This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. | |
| Mouse | |
| Colorimetric Microplate Reader | |
| C230052L06Rik,HGF/SF,NK1,NK2,SF,SF/HGF | |
| <12% | |
| Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-Mouse HGF precoated 96-well strip plate, lyophilized recombinant mouse HGF standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent (5X concentrated b | |
| 96 Tests | |
| Apoptosis | |
| 2°C to 8°C | |
| Mouse | |
| 4 hr. 45 min. |