Invitrogen™ Mouse anti-Human IgG4 Fc Secondary Antibody, HRP

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Storage and reconstitution: when stored desiccated at or below -20°C, the lyophilized powder is stable for at least one year. Reconstitute using 200 μL PBS, pH 7.2, to yield a 1 mg/mL stock solution containing 0.02% thimerosal. After reconstitution, store product at 4°C. For prolonged storage after reconstitution, add glycerol to a final concentration of 50% (v/v), aliquot, and store at or below -20°C. When stored properly, the solution is stable for approximately three months.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.