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Proteintech™ Mouse Adiponectin ELISA Kit

KE10044 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Adiponectin ELISA kit is to be used to detect and quantify protein levels of endogenous Adiponectin.

Brand:  Proteintech™ KE10044-96T


Product Code. 16851636

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Description

Description

  • The assay recognizes mouse Adiponectin. An antibody specific for Adiponectin has been pre-coated onto the microwells.
  • The Adiponectin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Adiponectin is added to detect the captured Adiponectin protein.
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm
  • Adiponectin, also known as Acrp30, AdipoQ, and GBP28, is an adipocyte-secreted protein that circulates in high concentrations in the serum and acts to increase insulin sensitivity.
  • Adiponectin modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation.
  • The serum concentration of adiponectin decreases in obese patients, opposite to other adipocytokines. In recent studies, serum adiponectin level progressively decreased in patients according to the severity of obesity, insulin resistance, diabetes mellitus and cardiovascular diseases.
  • Low serum adiponectin level may be a predictive factor for type 2 diabetes mellitus (T2DM) and other cardiovascular diseases.
Specifications

Specifications

BC028770
6.9 pg/mL
Sandwich ELISA, Double Antibody
Plasma, Serum
Mouse
Mouse
<10%
Blue ice
4.5 hr.
31.25 to 2000 pg/mL
ISO 13485
Coated ELISA Kit
DAB
11450
Solid
<10%
-20°C to +4°C
Q60994
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