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Invitrogen™ X-Rhod-1, AM, cell permeant - Special Packaging
Labeled calcium indicators that exhibit an increase in fluorescence upon binding Ca2+
Brand: Invitrogen™ X14210
Additional Details : Weight : 0.00525kg
Description
- Uses in many calcium signaling investigations, including measuring Ca2+ in cells and tissues
- Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells
- Fluorescence signal from these cells is generally measured using fluorescence microscopy
- Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
- In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+
- Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator
- Label (Ex/Em of Ca2+–bound form): X-rhod-1 (580/602nm)
- Fluorescence intensity increase upon binding Ca2+: >100 fold
- Kd for Ca2+ in the absence of Mg2+, in buffer: approximately 700nM
Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling
Order Info
Shipping Condition: Room temperature
Specifications
| Store in freezer -5°C to -30°C and protect from light. | |
| Cell Viability and Proliferation | |
| Dye | |
| Room Temperature |
| Fluorescent | |
| Fluorescence Microscope | |
| Fluorescent Dye-Based |
For Research Use Only. Not for use in diagnostic procedures.