Invitrogen™ Transferrin From Human Serum, Alexa Fluor™ 680 Conjugate

Transferrin is a monomeric serum glycoprotein (approx. 80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe³⁺ atoms via receptor-mediated endocytosis.

• Once iron-carrying transferrin proteins are inside endosomes, acidic environment favors dissociation of iron from transferrin-receptor complex
• Following release of iron, apotransferrin is recycled to plasma membrane, where it is released from its receptor to scavenge more iron
• Fluorescent transferrin conjugates can therefore be used with fluorescent LDL to distinguish the lysosomally directed and recycling endosomal pathways
• Experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy
• Label (Ex/Em): Alexa Fluor 594 (590/617)
• Amount: 15mg solid (contains 5mg of transferrin conjugate)
• Applications include: Image transferrin receptor dynamics using FRET; Observe receptor trafficking in live cells by confocal laser-scanning microscopy; Investigate events occurring during endosomal acidification; Measure transferrin receptor binding affinity in mammals and parasites

Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection