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Invitrogen™ PBFI, Tetraammonium Salt, cell impermeant
PBFI is potassium-sensitive molecule used measure intracellular K+ fluxes in animal cells and in plant cells and vacuoles
Brand: Invitrogen™ P1265MP
4920.24 DKK valid until 2024-12-14
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Description
- Although selectivity of PBFI for K+ is less than that of calcium indicators such as fura-2, it is sufficient for detection of physiological concentrations of K+ in presence of other monovalent cations
- Spectral response of PBFI upon ion binding permit excitation ratio measurements, and this indicator can be used with same optical filters and equipment used for fura-2
- Label (Ex/Em): PBFI (approximately 340,380/500nm)
- Lyophilized product may be dissolved in distilled water or aqueous buffer for use
- Product is typically loaded into cells by diffusion from patch pipet for correlated fluorescence imaging and electrophysiological recording
- Kd of PBFI for K+ is strongly dependent on whether Na+ is present, with value of 5.1mm in absence of Na+ and 44mm in solutions with combined Na+ and K+ concentration of 135mm (which approximates physiological ionic strength)
- Although PBFI is only 1.5-fold more selective for K+ than for Na+, this selectivity is often sufficient because intracellular K+ concentrations are normally about 10 times higher than Na+ concentrations
Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling, Sodium and Potassium Detection
Order Info
Shipping Condition: Room temperature
Specifications
| Store at room temperature and protect from light. | |
| Fluorescence Microscope | |
| Potassium Indicator | |
| Room Temperature |
| Fluorescence | |
| PBFI | |
| 1 mg |
For Research Use Only. Not for use in diagnostic procedures.